Hamoo, R., Al-Rubaye, F., Mustafa, N. (2020). Genotyping study of Fasciola gigantica isolated from cattle in Aqrah city, Iraq. , 34(1), 123-127. doi: 10.33899/ijvs.2019.125621.1108
Reedha N. Hamoo; Fouad S. Al-Rubaye; Nashaat G. Mustafa. "Genotyping study of Fasciola gigantica isolated from cattle in Aqrah city, Iraq". , 34, 1, 2020, 123-127. doi: 10.33899/ijvs.2019.125621.1108
Hamoo, R., Al-Rubaye, F., Mustafa, N. (2020). 'Genotyping study of Fasciola gigantica isolated from cattle in Aqrah city, Iraq', , 34(1), pp. 123-127. doi: 10.33899/ijvs.2019.125621.1108
Hamoo, R., Al-Rubaye, F., Mustafa, N. Genotyping study of Fasciola gigantica isolated from cattle in Aqrah city, Iraq. , 2020; 34(1): 123-127. doi: 10.33899/ijvs.2019.125621.1108

Genotyping study of Fasciola gigantica isolated from cattle in Aqrah city, Iraq

Iraqi Journal of Veterinary Sciences
Article 19, Volume 34, Issue 1, 2020, Pages 123-127    PDF (731.52 K)
Document Type: Research Paper
DOI: 10.33899/ijvs.2019.125621.1108
Authors
Reedha N. Hamoo1; Fouad S. Al-Rubaye1; Nashaat G. Mustafa* 2
1Department of Biology, College of Education for Girls, University of Mosul, Mosul, Iraq
2Department of Physiology, Biochemistry, and Pharmacology, College of Veterinary Medicine, University of Mosul, Mosul, Iraq
Abstract
This study was conducted to investigate the 18S rRNA gene of Fasciola gigantica obtained from the liver of cattle live in Aqrah city, Iraq. Fifty-nine Fasciola flukes were collected through routine investigation from livers of naturally infected local cattle (42 cows), from May to August 2017, at the central slaughterhouse of Aqrah city, Kurdistan region of Iraq, the flukes were washed by PBS and then fixed in ethanol. Genomic DNA was extracted, and a 560 bp fragment was amplified by PCR, subsequent by sequencing of PCR products. A remarkable result of this project was the deposition of our gene isolate in GenBank (Accession No. MG786553). However, it was confirmed by the sequence results that isolate species was F. gigantica, and interestingly our samples sequences have alignment match of 100% with many international isolates, without genetic mutations or variations. It is concluded that molecular study could be utilized for both diagnosis and differential diagnosis of parasites with huge precise. Also, an 18S rRNA gene is a perfect fragment for molecular study and phylogenetic analysis of F. gigantica, also our samples have 100% alignment match with universal isolates.
Keywords
18S rRNA gene; Fasciola gigantica; Iraqi cattle; Phylogenetic tree; Sequencing
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