Indirect Spectrofluorometric Determination of Enoxaparin Sodium in Pharmaceutical Formulation (Injection) by Ion Association Complex Formation with Acriflavine Dye

Aghwan, Mohamed Thamer; Al-talibi, Elham Sadullah

doi:10.33899/edusj.2019.125843.1001

	Indirect Spectrofluorometric Determination of Enoxaparin Sodium in Pharmaceutical Formulation (Injection) by Ion Association Complex Formation with Acriflavine Dye
Journal of Education and Science
Article 4, Volume 29, Issue 2, 2020, Pages 45-62 PDF (756.31 K)
Document Type: Research Paper
DOI: 10.33899/edusj.2019.125843.1001
Authors
mohamed thamer aghwan^* ¹; Elham Sadullah Al-talibi²
¹mosul university
²Department of Chemistry, College of Education for Pure Science, University of Mosul, Iraq.
Abstract
A simple, sensitive and selective spectrofluorometric method has been developed for the determination of enoxaparin sodium in bulk and dosage forms (injection). The method was based on the quantitative quenching effect of enoxaparin on the native fluorescence of acriflavine due to the formation of nonfluorescent supramolecular ion issociation complex between the studied drug and acriflavine dye in aqueous solution. The decrease of acriflavine fluorescence was observed at 506 nm after excitation at 402 nm. The relationship between quenching fluorescence intensity and concentration of enoxaparin sodium was linear in the range 0.05-20 µg/ml and with correlation coefficient 0.9990 and with LOD and LOQ 0.011 and 0.035 µg/ml respectively. The average recovery was 100.51% and RSD is less than 3.23%. The stoichiometry of enoxaparin to acriflavine was calculated a 1:4. The method was applied successfully to the determination of enoxaparin form injection samples and the results were in a good agreement with certified value and standard addition procedure.
Keywords
Spectrofluorometry,,; ,،,؛Acriflavine dye,,; ,،,؛Enoxaparin sodium,,; ,،,؛Ion association complex

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