Mikaeel, F., Al-Saeed, A. (2020). Molecular detection and seroprevalence of Toxoplasmosis in free range local chickens (Gallus domesticus) in Duhok province, Iraq. , 34(2), 247-252. doi: 10.33899/ijvs.2019.125885.1173
Farhad B. Mikaeel; Adel T. Al-Saeed. "Molecular detection and seroprevalence of Toxoplasmosis in free range local chickens (Gallus domesticus) in Duhok province, Iraq". , 34, 2, 2020, 247-252. doi: 10.33899/ijvs.2019.125885.1173
Mikaeel, F., Al-Saeed, A. (2020). 'Molecular detection and seroprevalence of Toxoplasmosis in free range local chickens (Gallus domesticus) in Duhok province, Iraq', , 34(2), pp. 247-252. doi: 10.33899/ijvs.2019.125885.1173
Mikaeel, F., Al-Saeed, A. Molecular detection and seroprevalence of Toxoplasmosis in free range local chickens (Gallus domesticus) in Duhok province, Iraq. , 2020; 34(2): 247-252. doi: 10.33899/ijvs.2019.125885.1173

Molecular detection and seroprevalence of Toxoplasmosis in free range local chickens (Gallus domesticus) in Duhok province, Iraq

Iraqi Journal of Veterinary Sciences
Article 5, Volume 34, Issue 2, 2020, Pages 247-252    PDF (547.41 K)
Document Type: Research Paper
DOI: 10.33899/ijvs.2019.125885.1173
Authors
Farhad B. Mikaeel* 1; Adel T. Al-Saeed* 2
1Department of Microbiology, College of Medicine, University of Duhok, Duhok, Iraq
2Department of Microbiology, College of Medicine, Duhok University, Duhok, Iraq
Abstract
Toxoplasmosis is a cosmopolitan zoonotic parasitic disease of mammals and birds; human infection occurs through consumption of raw or undercooked meat. Little was known about the infection rate of T. gondii among free range local chickens (Gallus domesticus) in Duhok province. Therefore, the present study was carried out to determine the infection rate in Duhok province by using ELISA (IgG) and conventional PCR. A total of 368 blood samples were collected from free range local chickens distributed in five different areas of Duhok province during the period from November 2016 to March 2017. The collected blood samples were from different sexes (hens and cocks) and from different age groups (less than 6 months and older than 6 months). The data found that the total infection rate was (84 / 368) 22.8% by ELSIA. The presence of the infection was confirmed by PCR and DNA sequencing. In this study, there were differences from area to area in the infection rates, the highest rate was reported in Semel district at 33.7% which was significantly (p
Keywords
chickens; PCR; ELISA; Toxoplasma gondii, Duhok
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