Metabolic changes of lipids & proteins during aging in terms of rooting response in mung bean (Phaseolus aureus Roxb.) cuttings

I. Merhij, Evan; M. Yasser, Oda; I. Shaheed, Abdullah

	Metabolic changes of lipids & proteins during aging in terms of rooting response in mung bean (Phaseolus aureus Roxb.) cuttings
Iraqi National Journal Of Chemistry
Article 1, Volume 0, Issue 34, 2014, Pages 347-354
Authors
Evan I. Merhij; Oda M. Yasser; Abdullah I. Shaheed
Abstract
Abstract Metabolic changes of lipids & proteins which occurs during aging of mung bean (Phaseolus aureus) cuttings were studied. The decline in rooting response was coincided with the decline of lipid & protein contents and verified by increasing the activity of lipoxygenase & protease respectively. The data revealed the followings:- 1. A decline in rooting response of aged cuttings (held in distilled water for 3 days) compared to fresh cuttings. 2. A significant increases in malondialdehyde (MDA) content by (88%), activity of lipoxygenase by (277.2%) and activity of protease by (212.1%). Whereas the proteins & antioxidants content (e.g. glutathione & ascorbate) were declined in aged cuttings compared to fresh cuttings. 3. For controlling aging phenomenon, the cuttings were kept in anise (Pimpinella anisum L.) seeds extract (1%), cinnamic acid (10-3M) & solutions of volatile oils, which led to stop the processes that occurs during aging in terms of rooting response by stopping the increase in MDA content, lipoxygenase activity & protease activity, and maintaining protein & lipid contents as well as glutathione & ascorbate contents. The discussion was focused on enzymes that associated with lipid peroxidation & the free radicals that produced by this process has increased during aging in terms of its final product (MDA). In addition the role of ascorbic acid and glutathione as antioxidants that involved in antioxidant defense mechanisms in terms of adventitious root formation in cuttings were assayed.

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