Indirect Spectrophotometric Determination of Paracetamol Via Decolorization of Eriochrom Black-T With N-Bromosuccinimide

Al-Sabha, Theiaa; Al-Gubouri, Zahraa Jameel

doi:10.33899/edusj.2020.126846.1060

	Indirect Spectrophotometric Determination of Paracetamol Via Decolorization of Eriochrom Black-T With N-Bromosuccinimide
Journal of Education and Science
Article 19, Volume 29, Issue 3, 2020, Pages 246-256 PDF (656.15 K)
Document Type: Research Paper
DOI: 10.33899/edusj.2020.126846.1060
Authors
Theiaa Al-Sabha^* ¹; Zahraa Jameel Al-Gubouri²
¹Department of Chemistry, College of Education for Pure Science, University of Mosul
²Chemistry department, College of Education for pure science, University of Mosul, Mosul, Iraq
Abstract
A simple, precise and sensitive indirect spectrophotometric method is described for the assay of Paracetamol in its pure form and pharmaceutical formulations in the aqueous medium. The method is based on the oxidation of the Paracetamol with an excess of N-Bromosuccinimide (NBS) in alkaline medium and the residual oxidizing agent bleaches the purple-colored Eriochrom black-T (EBT) to colorless species which is measured at 516 nm at room temperature. Calibration graph is linear over 0.5-9 µg mL-1 and molar absorptivity is 3.7×104 L.mol-1.cm-1. The detection and quantification limits were 0.068 and 0.229 µgmL-1 respectively. The accuracy (Average recovery %) is 98.57, and Precision (RSD) is ≤ 1.5. No interference effect has been observed from the excipients that exist in drug formulations. The method has been applied successfully in the determination of the Paracetamol in its commercial formulations (injection, syrup, and tablet), and compared favorably with other spectrophotometric methods used different reagents. The reaction mechanism for the oxidation of Paracetamol and EBT was postulated.
Keywords
Spectrophotometry,,; ,،,؛EBT,,; ,،,؛Paracetamol,,; ,،,؛Oxidation,,; ,،,؛NBS

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