Kinetic and inhibitory study of partially purified lipoxygenase from epilepsy patients serum

Al-Fayyad, Ahmed; Al-lehebe, Nashwan Ibrahem

doi:10.33899/edusj.2020.127778.1093

	Kinetic and inhibitory study of partially purified lipoxygenase from epilepsy patients serum
Journal of Education and Science
Article 18, Volume 30, Issue 1, 2021, Pages 58-71 PDF (989.51 K)
Document Type: Research Paper
DOI: 10.33899/edusj.2020.127778.1093
Authors
Ahmed Al-Fayyad^* ¹; Nashwan Ibrahem Al-lehebe²
¹university of mosul
²Chemistry Dep. College of Education for Pure Sciences Mosul University
Abstract
Abstract This research was included partially purification of lipoxygenase (LOX) from serum of patients with epilepsy using ammonium sulfate precipitation, dialysis and ion-exchange chromatography with specific activity (0.751 U / mg) , (0.935 U/mg) and (2.60 U/mg) respectively . Optimum conditions for enzyme activity were determined. The best enzyme activity was showed at 7 minutes of incubation time , 3 minutes of reaction time, pH = 7, temperature 40Cº and finally substrate concentration (linoleic acid) was (1.2 mmol/L) . By applying Michaelis-Menten and Lineweaver-Burk Equations, Michaelis-Menten constant (km) and Vmax values were found 0.3 (mM) and (0.9 U/ml) respectively. Also this study included the effect of some antiepileptic drugs such as valproic acid , carbamazepine and acetozolamide. Acetazolamide showed the highest inhibition of lipooxygenase activity(96.2٪) , valproic acid (95.6) and Carbamazapine (95.1٪). Inhibition type was studied and the result showed noncompetitive inhibition by using Lineweaver-Burke plot for all above drugs.
Keywords
Epilepsy,,; ,،,؛Lipoxygenase,,; ,،,؛inhibition,,; ,،,؛purification,,; ,،,؛antiepileptic drugs

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