Investigation of the Ability of some Bacteria Isolated from Intravenous Catheters to form Biofilms and the Effectiveness Ability of some Substances in Removing them

Mahmmod, Raad M.; Al-Rawi, Amera M.

doi:10.33899/rjs.2020.166315

	Investigation of the Ability of some Bacteria Isolated from Intravenous Catheters to form Biofilms and the Effectiveness Ability of some Substances in Removing them
Rafidain Journal of Science
Article 8, Volume 29, Issue 3, 2020, Pages 14-26 PDF (598.22 K)
Document Type: Research Paper
DOI: 10.33899/rjs.2020.166315
Authors
Raad M. Mahmmod^* ¹; Amera M. Al-Rawi²
¹Al-Hamdanyya General Hospital/ Directorate of Health Nineva/ Ministry of Health
²Department of Biology/ College of Sciences/ University of Mosul
Abstract
The research included investigating some of the contaminated bacteria of the Cervical Vein Catheter (CVC) devices in patients with dialysis in Ibn Sina Teaching Hospital (alternative site), then testing the ability of the isolated bacteria to form the biofilms in two ways, the congo red agar and the tube method, as well as evaluating the effectiveness of some substances to remove the biofilms The results showed that the contaminated bacteria of the cervical venous catheter apparatus included: Staphylococcus aureus, Staphylococcus haemolyticus and Burkholderia cepacia. The current study also showed that the ability of the pathogenic bacteria above to form the biofilms by Tube Method came at 3,2 and 0, bacteria respectively, and its ability to the composition of the biofilms by the Congo Red Agar Method came at 2,2 and 0, bacteria respectively. The results also showed the high ability of Vancomycin and Meropemen 10% for each one, and Alum 20% as well as natural lemon juice undiluted to remove the biofims formed by the Staphylococcus aureus bacterium. As for the other materials that were used, they did not show any ability to remove the biofilms.
Keywords
Cervical Vein Catheter; biofilm; Staphylococcusaureus; Bi

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