Thin layer chromatographic investigation of some veterinary drugs

Hamed, Zena S.; Yahya, Basil M.

doi:10.33899/ijvs.2012.168712

	Thin layer chromatographic investigation of some veterinary drugs
Iraqi Journal of Veterinary Sciences
Article 28, Volume 26, Suppl. II - Serial Number 3, 2012, Pages 53-55 PDF (177.58 K)
Document Type: Research Paper
DOI: 10.33899/ijvs.2012.168712
Authors
Zena S. Hamed¹; Basil M. Yahya²
¹Department of Pharmacology and Toxicology, College of Pharmacy. University of Mosul, Mosul, Iraq
²Department of Pharmacology, College of Pharmacy, University of Mosul, Mosul, Iraq
Abstract
A simple technique, using solvent extraction and thin layer chromatography for the detection and separation of a number of commonly prescribed veterinary drugs was studied. Blood and urine have been considered as possible biological samples taken from the animals. The extraction of each sample was done according to the type of the drug as it is acidic or basic, then the extract was spotted on TLC plate by capillary tube after lining the starting line first. Separations of the components of the samples were developed after running the plate in a tight lid tank containing solvent (mobile phase). The identification of separated spots were carried out after lining the end line for the solvent front then by spraying the plate with mercurous nitrate reagent for acidic drugs and acidic iodoplatinate or potassium permanganate reagent for basic drugs. The rate of flow value (R_f) for each compound was measured and the color of spots were determined. This work provides a simple and rapid qualitative technique for the detection of veterinary drugs used at therapeutic doses and in overdosage.
Keywords
Thin layer chromatography; Veterinary drugs; Drug analysis

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