Detection of AmpC Enzyme in Gram Negative Bacteria by Phenotypic and Molecular Methods

Shareef, Adeeba Younis; Saleem, Sahar Lukman

doi:10.33899/rjs.2021.168911

	Detection of AmpC Enzyme in Gram Negative Bacteria by Phenotypic and Molecular Methods
Rafidain Journal of Science
Article 6, Volume 30, Issue 3, 2021, Pages 16-26 PDF (897.14 K)
Document Type: Research Paper
DOI: 10.33899/rjs.2021.168911
Authors
Adeeba Younis Shareef¹; Sahar Lukman Saleem²
¹Biology Dept., College of Science, Mosul University, Mosul,IRAQ
²Biology Department, College of Science, University of Mosul
Abstract
In this study, the ability of some gram-negative bacteria isolated from different clinical samples to produce AmpC enzymes by phenotypic and molecular methods was investigated. The percentage of its occurrence was (40.6 %) by the use of cefoxitin disk as this antibiotic is used to screen for isolates that can produce this enzymes, the highest rate was for bacteria Pseudomonas aeruginosa (15.5 %) followed by Klebsiella pneumoniae (12.5 %), as for the three dimensional extraction method, the percentage of this enzyme reached (13.2 %), which depend on the use of crude enzymatic extracts instead of live microbial cells, the highest rate was for Pseudomonas aeruginosa (12.5 %) followed by Klebsiella pneumoniae (9.3 %), (37.5 %) by using disk antagonism test method which is one of the best methods for investigating these enzymes. As for molecular methods PCR assay was used to detect the bla_AmpC gene encoding for this enzyme, the highest percentage was for Klebceiala pneumoniae, Pseudomonas aeruginosa reached(13.3 %).
Keywords
AmpC Enzymes; Cram-negative bacteria; Cefoxtin

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