Effects of Menthol crystals aqueous extracts on salivary Streptococci and Mutans Streptococci in comparison to Chlorhexidine Gluconate (In vivo study)

AL-Casey, Mohammed; A. Al-Bazaz, Farah

	Effects of Menthol crystals aqueous extracts on salivary Streptococci and Mutans Streptococci in comparison to Chlorhexidine Gluconate (In vivo study)
Journal of baghdad college of dentistry
Article 1, Volume 23, Issue 2, 2011, Pages 119-123
Authors
Mohammed AL-Casey; Farah A. Al-Bazaz
Abstract
ABSTRACTBackground: Menthol is a chemical compound derived from mint family; it has been applied as a medicinalremedy for many conditions for more than 3 thousands years ago, including skin infections and burns, localizedpain, and in oral hygiene products and bad breath remedies like mouth wash and tooth paste. The aim of this studywas to test the effects of menthol crystals aqueous extract on the viable count of Salivary Streptococci and MutansStreptococci and on salivary flow rate and pH in comparison to chlorhexidine gluconate 0.2% and de-ionized waterin vivo.Materials and method: Menthol crystals aqueous extracts was prepared and estimated in mg/ ml, in which 1 ml ofalcohol was used to dissolve the specific amount of crystals, and then 99 ml of deionized water was added to forma solution of 100ml where as chlorhexidine gluconate used in this study as a control positive, de-ionized water wasused as a control negative. Stimulated salivary samples were collected from 15 volunteers (20-24 years) for five timeintervals (before rinsing, 1 minute, 15 minutes, 30 minutes and 1 hour after rinsing), they were divided into threegroups each group rinse with one of the tested agents for one minute). Mitis Salivarius Agar (MSA) media was usedfor isolation of Streptococci and Mitis Salivarius Bacitracin Agar (MSBA) media was used for isolation of MutansStreptococci. Colonies were counted using colony counter. Salivary pH was measured using digital pH meter andvolume of saliva was measured also.Results: Counts of bacteria were recorded at different time points. Results showed a reduction in the viability countsof Salivary Streptococci and Mutans Streptococci one minute immediately after rinsing which continue to decreaseafter one hour. The reduction in the viable count of Streptococci was found to be statistically significant after 15 and30 minutes of rinsing and highly significant after 1 hour, while for Mutans Streptococci, a significant reduction wasfound after 30 minutes of rinsing and highly significant after 1 hour. Salivary flow rates and pH were measured for thethree test and control groups of volunteers before and after rinsing for five time intervals. There was an increase inthe salivary flow rates and PH immediately after rinsing, and continue to increase after 30 minutes of rinsing, thendecrease after 1 hour to approximate the base line.Conclusion: Menthol proved to posses antimicrobial activities against salivary Streptococci and MutansStreptococci, and reduces their viable counts.Keyword: Mutans Streptococci, Menthol extracts, Chlorhexidine. (J Bagh Coll Dentistry 2011;23(119-123).

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