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PCR-BASED DETECTION OF PSEUDOMONAS FLUORESCENS IN COWS AND BUFFALOES RAW MILK | ||
| Basrah Journal of Veterinary Research | ||
| Article 16, Volume 15, Issue 1, 2016, Pages 194-208 PDF (296.73 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.23975/bjvetr.1970.170664 | ||
| Authors | ||
| Adnan M. Al –Rodhan* ; Hiba A . Nasear | ||
| Department of Microbiology, Collage of Veterinary Medicine, University of Basrah, Basrah, Iraq | ||
| Abstract | ||
| Investigations of the Psuedomonas fluorescens proteolysis activity is the basal objective of this study. To achieve this objective 16S rDNA and SM2F/SM3R primers were used in the amplification of DNA extracted from raw milk bacterial isolates. In the present study the 92 bacterial isolates , consisted of 42( cow raw milk isolates) and 50 (buffalo raw milk isolates) obtained from 240 cows and buffaloes raw milk samples (120) for each , were subjected to phenotypic and molecular identification. The results of phenotypic identification revealed that higher ratio (41.7 and42%) for bacterial isolates plating inoculation and biochemical tests based identification respectively was observed in buffaloes raw milk compare to the ratio observed in cows milk bacterial isolates (35 and 33.3% respectively), however the differences .between cows and buffaloes raw milk in concern to bacterial identification results considered to be not statistically significant ( P>0.05). The 16SrDNA and SM2F/SM3R based PCR results, revealed that the effect of breed(cow only) and age on the amplification results of the 16SrDNA and SM2F/SM3R gene product of P. fluorescens in cows and buffaloes raw milk is considered to be not statistically significant ( P>0.05). Depending on 16SrDNA based PCR results higher ratio of P. fluorescens identification (62.5 %) was observed in the native cows raw milk compare to crossbreed raw milk bacterial isolates ratio (57.7% ). SM2F/SM3R based PCR results showed 33.3 and 34% of raw milk P. fluorescens isolates of cows and buffaloes respectively had protease activity | ||
| Keywords | ||
| DNA; Milk; PCR | ||
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