Evaluation of Antimicrobial Activity of Plants Extract Against Bacterial Pathogens Isolated from Urinary Tract Infection among Males Patients

., Mais E Ahmed; Q. Al-lam, Makarim; ., Dina M Abd Ali

doi:10.33091/amj.2021.171060

	Evaluation of Antimicrobial Activity of Plants Extract Against Bacterial Pathogens Isolated from Urinary Tract Infection among Males Patients
Al- Anbar Medical Journal
Article 5, Volume 17, Issue 1, 2021, Pages 20-24 PDF (629.89 K)
Document Type: Original articles
DOI: 10.33091/amj.2021.171060
Authors
Mais E Ahmed .^* ; Makarim Q. Al-lam; Dina M Abd Ali .
Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq.
Abstract
Background: Urinary tract infection (UTI) is a considerable problem affecting the health of peopleeach year. It is caused by various Gram-positive (G+ve) and Gram-negative (G-ve) pathogens. Itis an important illness in the world affecting all age groups across their life span.Objectives: To identify the most common aerobic bacteria that cause UTIs and their antibioticsusceptibility and antimicrobial activity of plant extracts of the males’ patients.Materials and methods: The study involved 35 midstream urine samples from the male students(University of Baghdad, Baghdad, Iraq) with suspicious symptoms of UTI, during the period fromJanuary-March 2018. Each urine sample was cultured first on Mannitol Salt Agar and MacConkeyagar plates to differentiate between G+ve and -ve bacteria. The isolated bacteria were subjectedto certain antibiotics and 100% plant extract oils.Results: Out of 35 urine samples, there were 62 isolates. There were 34 (54.8%) G-ve and28 (45.2%) G+ve pathogens. Staph. epidermidis (n=17, 27.4%) was the most common isolatedpathogen, while P. aeruginosa (n=5, 8.1%) was the least isolated organism. Vancomycin wasthe most sensitive antibiotic for the Staph. epidermidis (52.9%) and Staph. epidermidis (45.5%).While oregano oil was more sensitive to Staph. epidermidis and Staph. aureus in 64.7% and 63.6%respectively. Imipenem was the most effective antibiotic for the three common G-ve isolates E. coli,K. Pneumoniae, and Enterobacter spp in 83.3%, 90%, and 85.7% respectively. Oregano oil was themost susceptible plant extract for E. coli (91.7%).Conclusion: Plants oils are potentially a good source of antimicrobial agents. Besides, the plantextract oils are cheaper than antibiotics. Therefore, they could be used in UTI medicine.
Keywords
antibacterial; Plant extract; Pathogenic bacteria; UTI

References
[1] B. Foxman, “Urinary tract infection syndromes: occurrence, recurrence, bacteriology, risk factors, and disease burden.,” Infect. Dis. Clin. North Am., vol. 28, no. 1, pp. 1–13, 2013. [2] E. S. Lestari et al., “Antimicrobial resistance among commensal isolates of Escherichia coli and Staphylococcus aureus in the Indonesian population inside and outside hospitals,” Eur. J. Clin. Microbiol. Infect. Dis., vol. 27, no. 1, p. 45, 2008. [3] H. Vashist and A. Jindal, “Antimicrobial activities of medicinal plants–Review,” Int J Res Pharm Biomed Sci, vol. 3, no. 1, pp. 222–230, 2012. [4] B. Tepe, D. Daferera, M. Sökmen, M. Polissiou, and A. Sökmen, “In vitro antimicrobial and antioxidant activities of the essential oils and various extracts of Thymus eigii M. Zohary et PH Davis,” J. Agric. Food Chem., vol. 52, no. 5, pp. 1132–1137, 2004. [5] M. Sohail, S. Sarwar, K. Rasool, and M. S. Iqbal, “Antimicrobial activity of various plant extracts against bacterial pathogens isolated from urinary tract infection patients,” Th. Pharma. Chem. J, vol. 1, no. 2, pp. 26–31, 2014. [6] R. Srinivasan et al., “Use of 16S rRNA gene for identification of a broad range of clinically relevant bacterial pathogens,” PLoS One, vol. 10, no. 2, 2015. [7] S. Rath, D. Dubey, M. C. Sahu, N. K. Debata, and R. N. Padhy, “Antibacterial activity of 25 medicinal plants used by aborigines of India against 6 uropathogens with surveillance of multidrug resistance,” Asian Pacific J Trop Biomed, vol. 2, pp. S846-54, 2012. [8] M. H. Mourad, S. S. Abdel-Rahman, M. M. Elaasser, N. A. Safwat, and M. Y. Ibrahim, “Antibacterial activity of certain medicinal plant and their essential oils on the isolated bacteria from UTI patients,” Int. J. Adv. Res, vol. 4, no. 12, pp. 1510–1530, 2016. [9] J. Randon, L. Maret, and C. Ferronato, “Gas chromatography–mass spectroscopy optimization by computer simulation, application to the analysis of 93 volatile organic compounds in workplace ambient air,” Anal. Chim. Acta, vol. 812, pp. 258–264, 2014. [10] Y. Huang, S.-H. Ho, H.-C. Lee, and Y.-L. Yap, “Insecticidal properties of eugenol, isoeugenol and methyleugenol and their effects on nutrition of Sitophilus zeamais Motsch.(Coleoptera: Curculionidae) and Tribolium castaneum (Herbst)(Coleoptera: Tenebrionidae),” J. Stored Prod. Res., vol. 38, no. 5, pp. 403–412, 2002. [11] B. A. Forbes, D. F. Sahm, and A. S. Weissfeld, “Bailey and Scott’s Diagnostic Microbiology. 12 [sup] th ed,” Missouri: Mosby Elsevier, p. 779, 2007. [12] J. F. MacFaddin, “Biochemical Tests for Identification of Medical Bacteria, Williams and Wilkins,” Philadelphia, PA, p. 113, 2000. [13] R. B. Nurullaev, “The role of asymptomatic bacteriuria in epidemiologic study of the urinary tract infection,” Likars’ ka Sprav., no. 7, pp. 23–25, 2004. [14] K. P. Devkota and I. C. Dutta, “Antibacterial activities of commercially traded herbs used in traditional medicines in communities of Doti district,” Nepal, Rep. Submitt. to IUCN, Kathmandu, Nepal, pp. 1–13, 2001. [15] E. J. Baron and S. M. Finegold, “Microorganisms encountered in the urinary tract,” Bailey Scott’s diagnostic Microbiol. 9th Ed. St. Louis, Missouri Mosby Publ., 1994. [16] H. Y. Serrheed and E. J. Mohammed, “Hemostatic changes in patients with chronic renal failure,” J. Fac. Med. Baghdad, vol. 60, no. 2, pp. 85–88, 2018. [17] E. Magliano et al., “Gender and age-dependent etiology of community-acquired urinary tract infections,” Sci. world J., vol. 2012, 2012. [18] G. Beyene and W. Tsegaye, “Bacterial uropathogens in urinary tract infection and antibiotic susceptibility pattern in jimma university specialized hospital, southwest ethiopia,” Ethiop. J. Health Sci., vol. 21, no. 2, pp. 141–146, 2011. [19] A. C. C. Lee et al., “Urinary tract infections in pregnancy in a rural population of Bangladesh: population-based prevalence, risk factors, etiology, and antibiotic resistance,” BMC Pregnancy Childbirth, vol. 20, no. 1, p. 1, 2020. [20] S. A. Ibrahim, D. A. Mohamed, and S. K. Suleman, “Microbial causes of urinary tract infection and its sensitivity to antibiotics at Heevi pediatric teaching hospital/Duhok City,” Med. J. Babylon, vol. 17, no. 1, pp. 109–114, 2020. [21] S. S. Tankhiwale, S. V Jalgaonkar, S. Ahamad, and U. Hassani, “Evaluation of extended spectrum beta lactamase in urinary isolates,” Indian J Med Res, vol. 120, no. 6, pp. 553–556, 2004. [22] G. A. Ouno et al., “Isolation, identification and characterization of urinary tract infectious bacteria and the effect of different antibiotics,” J. Nat. Sci. Res. III, vol. 6, pp. 150–159, 2013. [23] S. W. Wossa, T. Rali, and D. N. Leach, “Volatile chemical constituents of three Ocimum species (Lamiaceae) from Papua New Guinea,” South Pacific J. Nat. Appl. Sci., vol. 26, no. 1, pp. 25–27, 2008. [24] J. Tibyangye, M. A. Okech, J. M. Nyabayo, and J. L. Nakavuma, “In vitro antibacterial activity of Ocimum suave essential oils against uropathogens isolated from patients in selected hospitals in Bushenyi district, Uganda,” Br. Microbiol. Res. J., vol. 8, no. 3, p. 489, 2015. [25] S. SHARMA, S. SINGH, J. BOND, A. SINGH, and A. RUSTAGI, “Evaluation of antibacterial properties of essential oils from Clove and Eucalyptus,” Evaluation, vol. 7, no. 5, 2014. [26] J. Coccimiglio, M. Alipour, Z.-H. Jiang, C. Gottardo, and Z. Suntres, “Antioxidant, antibacterial, and cytotoxic activities of the ethanolic Origanum vulgare extract and its major constituents,” Oxid. Med. Cell. Longev., vol. 2016, 2016. [27] S. Xiao, P. Cui, W. Shi, and Y. Zhang, “Identification of essential oils with strong activity against stationary phase uropathogenic Escherichia coli,” bioRxiv, p. 702951, 2019.
Statistics Article View: 1,027 PDF Download: 460