TalibHumeidy, I. (2019). Determination of Doxycycline Hyclate in pharmaceutical samples via Oxidative Coupling Reaction using Spectrophotometric method. , 12(2), 29-36. doi: 10.37652/juaps.2022.171782
Israa TalibHumeidy. "Determination of Doxycycline Hyclate in pharmaceutical samples via Oxidative Coupling Reaction using Spectrophotometric method". , 12, 2, 2019, 29-36. doi: 10.37652/juaps.2022.171782
TalibHumeidy, I. (2019). 'Determination of Doxycycline Hyclate in pharmaceutical samples via Oxidative Coupling Reaction using Spectrophotometric method', , 12(2), pp. 29-36. doi: 10.37652/juaps.2022.171782
TalibHumeidy, I. Determination of Doxycycline Hyclate in pharmaceutical samples via Oxidative Coupling Reaction using Spectrophotometric method. , 2019; 12(2): 29-36. doi: 10.37652/juaps.2022.171782

Determination of Doxycycline Hyclate in pharmaceutical samples via Oxidative Coupling Reaction using Spectrophotometric method

Journal of University of Anbar for Pure Science
Article 7, Volume 12, Issue 2, 2018, Pages 29-36    PDF (556.29 K)
Document Type: Research Paper
DOI: 10.37652/juaps.2022.171782
Author
Israa TalibHumeidy
Dept. of Chemical Engineering/ College of Engineering / University of Tikrit
Abstract
This study involves the development of spectrophotometric method for determination of microgram a suggested mounts of doxycycline Hyclate (DCH) via oxidative coupling with 2,4,dinitrophenylhydrazine reagent (2,4-DNPH) in presence of potassium periodate(KIO4) in the alkaline medium (pH 12.6) to form a red colored dye which soluble in water and gives the highest intensity of absorption at λmax 528 nm. The method has been obeyed Beer's law in the concentration range 5-60 μg.ml-1with the molar absorptivity of 10771 L. mol-1. cm-1, Sandel Index value 0.0476 μg.cm-2 with the relative standard deviation of the method does not exceed its value 1.043 % and the correlation coefficient 0.998. The proposed method was applied successfully for the determination of doxycycline hyclate in its Pharmaceutical samples (Capsule).
 

 
Keywords
Spectrophotometric method; doxycycline Hyclate; 2; 4-DNPH
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