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Isolation and Identification of Klebsiella pneumoniae from Infants with Necrotizing Enterocolitis | ||
| Journal of University of Anbar for Pure Science | ||
| Article 1, Volume 13, Issue 1, 2019, Pages 1-6 PDF (503.87 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.37652/juaps.2022.171864 | ||
| Authors | ||
| Abdulkareem Hmood; Luma Zwain* | ||
| Department of Biology, Collage of Education for Pure Sciences Ibn Al-Haitham, Baghdad University, Baghdad, Iraq | ||
| Abstract | ||
| Sixty four clinical isolates were collected from children with Necrotizing Enterocolitis admitted to the Children's Protection Hospital at the Medical City in Baghdad. These isolates included (41) stool samples, (14) blood samples and (9) urine samples for the period from 29/1/2018 to 4/4/2018. All the samples were cultured on MacConkey agar and Blood agar for diagnosis. All isolates were identified depending on macroscopic, microscopic, and biochemical tests, with Vitek-2 compact system. Forty three isolates were obtained from all samples; (26) isolates as Klebsiella pneumoniae (60.46%), 8 isolates as Escherichia coli (18.60 %), 4 isolates as Pseudomonas aeruginosa (9.30%), 2 isolates as Klebsiella oxytoca (4.65%), 2 isolates as Enterobacter cloacae (4.65%) and 1 isolate as Proteus hauseri (2.32%).The results showed that K. pneumoniae was the predominant in the samples taken from infants infected with Necrotizing Enterocolitis . | ||
| Keywords | ||
| Klebsiella pneumoniae; Isolation; identification; Necrotizing Enterocolitis | ||
| References | ||
|
1- Nguyen , D. N. ; Stensballe , A. ; Lai , J . C. ; Jiang , P. ; Brunse , A. ; Li , Y. ; Sun , J . ; Mallard , C. ; Skeath , T. ; Embleton , N. D. ; Berrington , J . E. and Sangild , P. T. (2017) . Elevated Levels of Circulating Cell – free DNA and Neutrophil Proteins are Associated With Neonatal Sepsis and Necrotizing Enterocolitis in Immature Mice , Pigs and Infants . Innate Immunity . 23(6):524-536.
2- Coggins , S. A. ; Wynn , J. L. and Weitkamp , J- H . ( 2015). Infectious Causes of Necrotizing Enterocolitis . Clin Perinatol . 42(1) : 133-154
3- Sharmeen , R. ; Hossain , M. N. ; Rahman , M. M. ; Foysal , M. J . and Miah , M. F. (2012 ) . In – Vitro Antibacterial Activity of Herbal Aqueous Extract Against Multi – Drug Resistant Klebsiella sp. Isolated from Human Clinical Samples . International Current Pharmaceutical Journal. 1(6) : 133-137.
4- Brenner , D. J. ; Krieg , N. R. and Staley , J. R. (2005) . Bergey's Manual of Systematic Bacteriology , Volume 2: the Proteobacteria , Part B: The Gamma Proteobacteria . New Yourk , Springer .
5- Ryan , K. J. and Ray , C. G. (2004). Sherris Medical Microbiology . 4th ed Library of congress.
6- Podschun , R. and Ullman , U . (1998) Klebsiella spp . As Nosocomial Pathogens Epidemiology , Taxonomy , Typing Methods , and Pathogenicity Factors . Clinical Microbiology Reviews . 11(4) : 589 – 603 .
7- Debby , B. D. ; Ganor , O. ;Yasmin M . ; David , L . ; Nathan , K. ; Ilana , T. ; Dalit , S. ; Smollan , G. and Galia , R. (2012). Epidemiology of Carbapenem Resistant Klebsiella pneumoniae Colonization in an Intensive Care Unit . Europe Journal Clinical Microbiology Infectious Disease . 31:1811-1817.
8- Singhai , M. ; Malik , A . ; Shahid , M. ; Malik , M. A. and Goyal , R . (2012) . A Study on Device – Related Infections With Special Reference to Biofilm Production and Antibiotic Resistance . Journal of Global Infectious Diseases . 4(4): 193-198.
9- Paczosa , M. K. and Mecsas , J. (2016). Klebsiella pneumoniae : Going on the Offense With a Strong Defense . Microbiology Molecular Biology Reviews . 80:629-661.
10- Ahmed , T. A. ; Haroun , M . ; Hussein , A. A. ; El-Ashry , E. H. and El-Sayed , L . H . (2012) . Development of a New Trend Conjugate Vaccine For the Prevention of Klebsiella pneumoniae . Infectious Disease Reports . 4: e33. P . 128 –133 .
11- Li , B. ; Zhao , Y . ; Liu , C . ; Chen , Z . and Zhou , D. (2014) . Molecular Pathogenesis Of Klebsiella pneumoniae. Future Microbiology. 9 (9): 1071-1081.
12- Wu , C. C. ; Huang , Y.J . ; Fung , C.P. and Peng , H.L. (2010). Regulation of the Klebsiella pneumoniae Kpc Fimbriae by the Site-Specific Recombinase Kpcl. Microbiology. 156:1983-1992.
13- Struve , C. ; Bojer , M. and Krogfelt , K. A. (2009). Identification of a Conserved Chromosomal Region Encoding Klebsiella pneumoniae Type 1 and Type 3 Fimbriae and Assessment of the Role of Fimbriae in Pathogenicity . Infection and Immunity. P. 5016-5024.
14- Hansen , D. ; Mestre , F. ; ALBerti , S. ; Hernandez-Alles , S. ; Varez , D. ; Domenech- Sanchez , A. ; Gil , J. ; Merino , S. ; Tomas , J. M . and Benedi , V. J. (1999). Klebsiella pneumoniae Lipopolysaccharide O Typing : Revision of Prototype Strains and O-Group Distribution among Clinical Isolates from Different Sources and Countries. Journal of Clinical Microbiology. P. 56-62.
15- Seifi , K. ; Kazemian , H. ; Heidari , H. ; Rezagholizadeh , F. ; Saee , Y. ; Shirvani , F. and Houri , H. (2016). Evaluation of Biofilm Formation Among Klebsiella pneumoniae Isolates and Molecular Characterization by ERIC –PCR. Jundishapur Journal Microbiology . 9(1) : e 30682.
16- Schroll , C. ; Barken . K. B. ; Krogfelt , K. A. and Struve , C. (2010). Role of Type 1 and Type3 Fimbriae in Klebsiella pneumoniae Biofilm Formation . Bio. Med. Central Microbiology . 10 : 179 .
17- Grishin , A. ; Papillon , S. ; Bell , B. ; Wang , J . and Ford , H. R . (2013). The Role of the Intestinal Microbiota in the Pathogenesis of Necrotizing Enterocolitis . Semin Pediatric Surgery . 22(2): 69-75.
18- Warner , B. B. ; Deych , E. ; Zhou , Y. Z. ; Hall – Moore , C. ; Weinstock , G. M. ; Sodergren , E . ; Shaikh , N. ; Hoffmann , J. A. ; Linneman , L. A. ; Hamvas , A. ; Khanna , G. ; Rouggly – Nickess , L. C. et al (2016).Gut bacteria dysbiosis and necrotizing enterocolitis in very low brith weight infants :A PROSPECTIVE CASE CONTROL STUDY .LANCET , 387:1928-1936
19- Raveh – Sadka , T. ; Thomas , B. ; Singh , A. ; Firek , B. ; Brook , B. ; Castelle , C. J . ; Sharon , I . ; Baker , R. ; Good , M. ; Morowitz , M. J . and Banfield , J . F. ( 2015) . Gut Bacteria are Rarely Shared by Co – Hospitalized Premature of NEC Development. e Live Science . 4 : e 5477. | ||
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