Detection of fimH virulence gene in uropathogenic Escherichia coli Using PCR

Fadhil, Rawan Amer

doi:10.37652/juaps.2022.172335

	Detection of fimH virulence gene in uropathogenic Escherichia coli Using PCR
Journal of University of Anbar for Pure Science
Article 12, Volume 14, Issue 2, 2020, Pages 1-4 PDF (1.58 M)
Document Type: Research Paper
DOI: 10.37652/juaps.2022.172335
Author
Rawan Amer Fadhil^*
Microbiology Department/College of Medicine, Al-Mustansiriyah University, Iraq
Abstract
Urinary tract infections (UTIs) are one of main health problems caused by many microorganisms, including uropathogenic Escherichia coli (UPEC). The virulence factors are important in colonization of UPEC, extra-intestinal survival, and creation of cytopathic effects. In addition, the expression of special virulence factors of UPEC can contribute to uropathogenicity, as well as worsening of UTIs. In this study, 134 isolates of uropathogenic E. coli were collected from Baghdad/Iraq through March 2018 to April 2019. UPEC were identified by culturing on Hardy chromo UTI agar and confirmed at molecular level by using uidA housekeeping gene using real time polymerase chain reaction.The prevalence of fimH gene was studied among 134uropathogenic Escherichia coli of patients attended Using polymerase chain reaction assay, Visualization of fimH gene (bp) by 1.5% agarose gel analysis. By comparing the bands in a sample to the DNA ladder, their approximate sizes can be determining. For instance, the bright band on the gel above 1000 base pairs (bp) in size for fimH. The intended gene was detected in 94.8% of the isolates and absence in 5.2%.of them.
Keywords
fimH; UPEC; Virulence genes; Single nucleotide polymorphisms

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