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Immunohistochemical Expression of Caspase 7 and Annexin V as Apoptosis Markers in Oral Squamous Cell Carcinoma (A clinicopathological study) | ||
| Journal of baghdad college of dentistry | ||
| Article 1, Volume 23, Issue 2, 2011, Pages 74-77 | ||
| Authors | ||
| Riyadh O. Alkaisi; Shatha A. Jabur | ||
| Abstract | ||
| ABSTRACTBackground: Oral squamous cell carcinoma (HNSCC) is an aggressive and lethal malignancy .It is an epithelialmalignancy with morphologic features of squamous cell differentiation without additional features suggestive ofother differentiated tissues. Caspase 7 is a caspase involved in execution phase of apoptosis. The genetic alterationof caspase 7 might be involved in the development of human cancer. Annexin V belongs to a family ofphospholipids binding proteins. It binds in the presence of Ca+2 ions with high affinity to negatively chargedphospholipids like phosphatidylserine(PS) exposed on cell surface during apoptosis.Materials and Methods: The study is a prospective one in which twenty cases of oral squamous cell carcinoma andeleven retrospective of paraffin embedded formalin fixed of oral squamous cell carcinoma blocks. H&E stain wasdone for each block for reassessment of histological examination. An immunohistochemical staining done by usingmonoclonal antibodies for caspase-7 and annexin V. The aim of the study: The aim of the study is to evaluate theimmunohistochemical expression of caspase 7 and Annexin V as apoptosis markers in correlation with variousclinicopathological parameters in oral squamous cell carcinomaResults: The results of 31 oral squamous cell carcinoma cases included in this Study. Age group of studying sampleswas more than 50 years. Immunohistochimical expressions were as follows in all cases of oral squamous cellcarcinoma for caspase 7 and Annexin V tumor markers: The expression of caspase-7 was weak positive in 12 cases(38%), strong positive in 11 cases (35%) and 8 (25%) cases were negative. The expression of Annexin V was weakpositive 16 cases (51%), strong positive 10 cases (32%) and 5 cases (16%) were negative.Statistical result for caspase 7 and AnnexinV with clinlinicopathological parameters was no significant correlationbetween them.Conclusions: Immunohisto chemical expression were observed in studying samples of oral squamous cell carcinomaof both annexin V and caspase 7, however, statistically non significant correlation with all clinicopathologicalfindings were found and between both markers.Key words: oral squamous cell carcinoma, apoptosis, caspase 7, annexin V. (J Bagh Coll Dentistry 2011;23(74-77). | ||
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