Spectrophotometric Assay of Mesalazine in Pharmaceutical Preparations Via Oxidative coupling reaction with o-cresol and sodium metaperiodate

Salih, Elham S; Al-Enizzi, Mohammed

doi:10.33899/edusj.2020.172815

	Spectrophotometric Assay of Mesalazine in Pharmaceutical Preparations Via Oxidative coupling reaction with o-cresol and sodium metaperiodate
Journal of Education and Science
Article 21, Volume 29, Issue 1, 2020, Pages 279-292 PDF (533.78 K)
Document Type: Research Paper
DOI: 10.33899/edusj.2020.172815
Authors
Elham S Salih^* ; mohammed Al-Enizzi
Department of Chemistry, College of Education for pure science, University of Mosul, Mosul, Iraq
Abstract
A simple, straightforward, rapid, sensitive and an accurate UV-VIS spectrophotometric method was suggested for the determination of mesalazine in bulk sample and in dosage forms. The method is relying on the oxidation of mesalazine by sodium metaperiodate followed by coupling with o-cresol in alkaline medium to form a blue indophenol dye which absorbs at 645 nm. Beer's law is obeyed in the concentration range 0.4- 12 µg/ml of the drug with good accuracy (average recovery) 101.38% and precision (RSD) is less than 3.5%. The molar absorptivity is 22435 l.mol^-1.cm^-1 with detection limit 0.101 µg/ml and 0.338 µg/ml as quantification limit. The recommended method is effectively applied to the determination of mesalazine in pharmaceutical dosages (tablets and capsule). The analytical results were well-matched with the standard method British pharmacopeia) and validated good accuracy and precision.
Keywords
Mesalazine; o-cresol; oxidative coupling reaction; Spectrophotometric

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