Using of Bathophenanthroline Reagent for Spectrophotometric Estimation of Acetylcysteine in its Pharmaceutical Preparations

Hasan, Shahad L.; Sultan, Saad Hasani

doi:10.33899/rjs.2022.172937

	Using of Bathophenanthroline Reagent for Spectrophotometric Estimation of Acetylcysteine in its Pharmaceutical Preparations
Rafidain Journal of Science
Article 10, Volume 31, Issue 1, 2022, Pages 21-33 PDF (662.01 K)
Document Type: Research Paper
DOI: 10.33899/rjs.2022.172937
Authors
Shahad L. Hasan^* ¹; Saad Hasani Sultan²
¹Department of Chemistry/ College of Science / University of Mosul
²Department of Chemistry Collage of Science University of Mosul, Mosul, Iraq
Abstract
A sensitive and rapid spectrophotometric method was suggested for the estimation of acetylcysteine in its pharmaceutical formulations. The proposed method was depended on the oxidation of the studied drug compound by iron (III) in aqueous medium, and then reaction of produced iron (II) with bathophenanthroline reagent to form a red color complex, which is stable and soluble in water and exhibits a maximum absorption at 534 nm. Beer^̛̛s law is obeyed over the concentration range of 0.25 to 10.0 µg.ml^-1 of acetylcysteine. The apparent molar absorptivity value is 2.35×10⁴ l.mol^-1.cm^-1 and Sandall^’s sensitivity index of 0.00694 µg.cm^{-2, a relative error of} -4.72 to 4.32% and a relative standard deviation of 0.627 to 3.54% depending on the concentration level. The proposed method was applied successfully to the determination of acetylcysteine in its pharmaceutical preparations as sachet (powder), capsule and injection.
Keywords
acetylcysteine; oxidation-reduction; bathophenanthroline; Spectrophotometric method

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