An Evaluation of Cytotoxicity of Iron Oxide Nanoparticles with Hydrogen Peroxide Endodontic Irrigant

Al-Mallah, Saraa; Al-Naimi, Ali

doi:10.33899/rden.2021.129103.1069

	An Evaluation of Cytotoxicity of Iron Oxide Nanoparticles with Hydrogen Peroxide Endodontic Irrigant
Al-Rafidain Dental Journal
Article 1, Volume 23, Issue 1, 2023, Pages 1-8 PDF (464.12 K)
Document Type: Research Paper
DOI: 10.33899/rden.2021.129103.1069
Authors
Saraa Al-Mallah^* ¹; Ali Al-Naimi²
¹Conservative department. College of Dentistry, University of Mosul, Iraq
²Conservative Department, College of Dentistry, University of Mosul, Iraq
Abstract
Aims: The aim of this research was to determine the cytotoxic effect of different concentrations of iron oxide nanoparticles (IONPs) in combination with hydrogen peroxide ( H₂O₂) in vitro as a potential endodontic irrigating solution. Materials and Methods: Cytotoxicity of IONPs at concentrations (8, 4, 2, 1, 0.5, and 0.25 mg/ml) with 3% H₂O₂ were evaluated on cultured Human Dermal Fibroblast (HDFn) cell line. Cytotoxicity was assessed after 10 minutes of exposure using the Mosmann’s Tetrazolium Toxicity (MTT) assay. Results: Tukey’s test indicated that no significant difference in cell viability was found at lower concentrations (0.2, 0.5, 1, and 2 mg/ml). While at a concentration of 4 and 8 mg/ml, the cell viability was not significantly different from each other whereas, significantly different from previous concentrations. Conclusion: From the gained data, it can be deduced that IONPs+H₂O₂ had almost no cytotoxic effect on HDFn cell viability at concentrations 0.2, 0.5, 1, and 2 mg/ml, while a moderate decrease of survival has been observed at concentrations 4 and 8 mg/ml.
Keywords
Cytotoxicity; human dermal fibroblast cells; hydrogen peroxide; Iron oxide; Irrigating solutions

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