Spectrophotometric Estimation of Clonazepam as Pure Form and in its Pharmaceutical Formulation (Tablet) Using Alizarin Red S

Yassin, Rabee M. A.; Othman, Nabeel S.

doi:10.33899/rjs.2022.174271

	Spectrophotometric Estimation of Clonazepam as Pure Form and in its Pharmaceutical Formulation (Tablet) Using Alizarin Red S
Rafidain Journal of Science
Article 3, Volume 31, Issue 2, 2022, Pages 22-33 PDF (855.83 K)
Document Type: Research Paper
DOI: 10.33899/rjs.2022.174271
Authors
Rabee M. A. Yassin¹; Nabeel S. Othman^* ²
¹Department of Chemistry/ College of Science/ University of Mosul
²Department of Chemistry/ College of Science/ Mosul University
Abstract
An easy and sensitive spectrophotometric method has been suggested for the assay of clonazepam (CLZM) as pure form and in its formulation as tablets. The present method based on the reaction of CLZM with alizarin red S reagent (ALRS) via proton transfer reaction to form a colored solution with a maximum absorption at wavelength of 528 nm and the optimal conditions for the reaction were studied. The linearity was within the concentration range from 1 to 30 µg/ml, with an R² value (determination coefficient) of 0.9882 and the sensitivity express via the molar absorption of 7.04x10³l mol^-1.cm^-1, while Sandell's sensitivity index has been determined and equal to 0.0448 µg/ cm².The percentage of relative standard deviation (RSD%) which express the precision of the method, percentage of relative error (RE%) express the accuracy, the LOD and LOQ also have been estimated. The application of the suggested method to the determination of CLZM in tablet gave satisfactory results.
Keywords
clonazepam; alizarin red S reagent; Spectroscopy; proton transfer

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