MOLECULAR DETECTION OF NUC AND SEA GENES OF STAPHYLOCOCCUS AUREUS ISOLATED FROM COW AND SHEEP MEAT IN BASRAH CITY.

Mazaal, Muhannad; Ibrahim, Hanaa; Mater, Abbas

doi:10.23975/bjvetr.2031.175863

	MOLECULAR DETECTION OF NUC AND SEA GENES OF STAPHYLOCOCCUS AUREUS ISOLATED FROM COW AND SHEEP MEAT IN BASRAH CITY.
Basrah Journal of Veterinary Research
Article 13, Volume 20, Issue 1, 2021, Pages 138-147 PDF (1.12 M)
DOI: 10.23975/bjvetr.2031.175863
Authors
Muhannad Mazaal¹; Hanaa Ibrahim^* ²; Abbas Mater³
¹Department of Microbiology, College of Veterinary Medicine,University of Basrah,Basrah,Iraq
²*Department of Microbiology, College of Veterinary Medicine,University of Basrah,Basrah,Iraq
³Department of Basic Sciences, College of Dentistry ,University of Thi Qar,Thi Qar,Iraq
Abstract
A total of 100 samples collected randomly from different butcher shops, restaurants and fast food places at Basrah Province (60 raw meats and 40 cooked spiced meat burger). Molecular detection of the nuc gene showed that 13(76.47%) out of 17 isolates from the cow meat were positive for this gene. However, only10 (52.63%) out of the 19 isolates from the sheep meat were positive for the nuc gene. The present study found that 9/13 isolates of cow meat samples were positive for sea gene. Regarding to sheep meat samples, the current study found that out of 10 isolates of S.aureus, 7(70%)isolates were positive for sea gene, without significant differences, and 3/8 (37.5%) of restaurants isolates were positive for sea gene. In the current study, the molecular detection showed that 5/9(55.56%)of fast food isolates were positive for sea gene. In the current study demonstrate that the meat is adequate medium for growth of S. aureus and production of enterotoxins.
Keywords
Gene; SEA; PCR

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