- All Journals
- Al-Qadisiyah University
- Basrah University
- Kirkuk University
- Mosul University
- The Iraqi Borad for Medical Specialization
- University of Anbar
- University of Technology
- Al Mustafa University
- Al-Zahraa University for Women
- Alnoor University
- Kunooz University
- Misan University
- Ninevah University
- Tikrit University
- University of Al-Hamdaniya
- University of Kerbala
Production of Glucose isomerase from local Isolate of Streptomyces sp. HM5 2-Optimization of enzyme production conditions by submerged cultures | ||
| Journal of Research Diyala humanity | ||
| Article 1, Volume 0, Issue 31, 2017, Pages 11-32 | ||
| Authors | ||
| M. O. Muhyaddin; H. A. Jabur; B. K. Dalaly | ||
| Abstract | ||
| This study was aimed to investigate the cultural requirements for production of glucose isomerase by locally isolated Streptomyces sp.HM5 The optimum conditions for production of the enzyme by submerged culture was achieved on broth medium containing 1.5% of xylose as a sole carbon source and inducer for enzyme production , peptone with 1% as a nitrogen source and 0.05% of each one of magnesium sulfate and cobalt chloride as a metal ions source with an initial pH of 7.5 during an incubation period of 72 hours at 35°C. This optimum conditions for enzyme activity attained 9.59 unit/ml it means the enzyme activity increased about 192% in comparation with those before using optimum conditions. | ||
| Keywords | ||
| Production of the enzyme glucose Oazomez; bacteria Streptomyces sp; Streptomyces sp | ||
|
Statistics Article View: 37 PDF Download: 5 |
||