Antibacterial activity of Opuntia ficus indica extraction and identification of some active compounds.

Aziz, Marwa; Hussien, Sabri Mohammed; Hassan, Abdulla Salih

doi:10.37652/juaps.2017.176991

	Antibacterial activity of Opuntia ficus indica extraction and identification of some active compounds.
Journal of University of Anbar for Pure Science
Article 35, Volume 9, Issue 2, 2015, Pages 102-111 PDF (2.21 M)
Document Type: Research Paper
DOI: 10.37652/juaps.2017.176991
Authors
Marwa Aziz^* ¹; Sabri Mohammed Hussien²; Abdulla Salih Hassan²
¹Department of Chemistry College of Science University of Anbar
²College of Medicine, University of Anbar
Abstract
Extraction of vegetative part of the plant Opuntia ficus indica was carried out by different polar solvents (i.e; Water, Methanol 60%, Absolute Methanol, Absolute Ethanol, Acetone, Ethyl acetate and Chloroform). Percentage of each extract was calculate and found as follow: H₂O > 60% Me OH > abs. Me OH > abs. Et OH > Acetone > Ethyl acetate > Chloroform. Some organic acids (i.e; Salicylic and Tannic acids) were separated from liquid extraction by High Performance Liquid Chromatography (HPLC) and Thin Layer Chromatography (T.L.C.) The percentage of their acids 24% and 43% respectively. Spectroscopic tools (U.V and I.R) were used to identify the structure of these acids. Oil of Opuntia ficus indica was obtained by Soxhlet. The percentage of this oil was 2.5%. Antibacterial activity was studied for different extracts from Opuntia ficus indica and oil in the growth of three undiagnosed isolates of Gram negative and positive bacteria by Agar-Well diffusion method. Results of antibacterial activity study appeared variable activity depending on the nature of solvent and the nature of polar compound used in extraction.

References
- محمد السعدی , "خفایا واسرار النباتات الطبیة والعقاقیر فی الطب القدیم والحدیث " عمان, الاردن, الطبعة العربیة 2006. 2- غسان حجاوی وحیاة حسین المسیمی ورولا محمد قاسم, " علم العقاقیر والنباتات الطبیة " عمان , دار الثقافة 2009 . 3- المرسومی صبری , عبدالله صالح ( 2012 ) – زیوت النباتات الطبیة لعلاج الامراض العصریة – مطبعة الاثیر للنشر– العراق. 4-السید، عبدالباسط ، "کنوز الطب الشعبی البدیل الوقایة والعلاج" ، الناشر دار لقمان – مصر ، 256 – 250 ، (2003). 5- عمیرة ، اسراء ، "علم العقاقیر الطبیة النظریة والعلمیة" ، دار البدایة للنشر والتوزیع – عمان ، (2005) , 115 , 82 40 – 33. 6- جبر, ریم محمود , الوجیز فی علم العقاقیر والنباتات الطبیة " عمان , الاردن , الطبعة العربیة الاولى (2007) . 7- شمس الدین, احمد " التداوی بالأعشاب والنباتات قدیما وحدیثا ". دار الکتب العلمیة. بیروت, لبنان (2000). 8- Al-Jugaimi, F., Ozcan, M. M. (2013). Determination of some mineral contents of prickly pear (Opuntia ficus-indica) seed flours, 185(5): 3659-3663. 9- Feugang, J.M., Konarski, P., Zou, D., Stintzing, F.C. and Zou, C.(2006). Nutritional and medicinal use of cactus pear (Opuntia spp.) cladodes and fruits-frontiers in Bioscience, 11,2574-2589. 10- Xian-Ke, Z., Xin, J., Feng-ying, L. (2010). Chemical analysis antioxidant activities in vitro of polysaccharide extracted from Opuntia ficus-indica Mill. Cultivated in China, Carbohydrate Polymer, 82(3):722-727. 11- Pretti, L., Bazzu, G., Serra, P. (2012). A novel method for the determination of ascorbic acid and antioxidant capacity in Opuntia ficus-indica using in vivo micro dialysis, Food Chemistry, 147:131-137. 12- البالانی,ماجد رشید مجید,"تاثیر المستخلصات النباتیة الخام وقلوید الفازیسینلنبات حلق السبع لشجیریAdhatoda Vasica ",رسالة ماجستیر ,کلیة العلوم, جامعة بغداد, بغداد (2003). 13- Duke , J. A; and A. A, Atchley "proximate analysis , In" , the hand book of plant science in agriculture. (Ed): B. R. chrwastie . CRC press , Boca Raton , FL (1984). 14- Cruickshank,R.;Duguid,J.P.;Marmion,B.P.And Sawain,R.H." Medical Microbiology" .12th ed , 2, Churchill Livingstone , New York (1975).
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