(Indirect Spectrophotometric Determination of Pyridoxine Hydrochloride Using Eriochrome Black-T)

Altaee, Zeinab Myaser; Shihab Al-Hammoodi, Intisar Adil

doi:10.33899/berj.2023.179256

	(Indirect Spectrophotometric Determination of Pyridoxine Hydrochloride Using Eriochrome Black-T)
College Of Basic Education Research Journal
Volume 19, Issue 2, June 2023, Pages 827-845 PDF (926.68 K)
Document Type: Research Paper
DOI: 10.33899/berj.2023.179256
Authors
zeinab myaser altaee^* ¹; Intisar Adil Shihab Al-Hammoodi²
¹Ninawa education
²Department of Chemistry , College of Education for Girls , University of Mosul , Mosul , Iraq
Abstract
The research included the description of a simple indirect spectrophotometric method for the determination of pyridoxine hydrochloride, which depended on the oxidation of pyridoxine with an amount of N-bromosuccinimide in the presence of hydrochloric acid and the excess amount of it oxidized the dye Eriochrome black-T, and the absorption was measured at 517 nm, and the molar absorption coefficient was 8 × 103 L / mol. cm, indicating the sensitivity of the method. And compliance with Beer's law for concentrations ranged from (2-14) micrograms. ML-1, the method was characterized by good accuracy and compatibility, with a recoverability of 99.07%. While the relative standard deviation was less than 0.63.The method was applied to pharmaceutical preparations of pyridoxine hydrochloride. So, what have been mentioned above represent clearly the importance of doing such researches in this simple and sensitive one. Researchers within the field of analytical chemistry can follow the same procedures with different dyes .ِ Also, other pharmaceutical preparations ( of local and international sources ) can be worked on holding a comparative study. So, things should be in this direction to reach the best results with low costs.
Keywords
spectrophotometric,,; ,،,؛pyridoxine hydrochloride,,; ,،,؛Eriochrome black-T,,; ,،,؛Beer's law

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