A. Jalal, R., E. Aziz, K. (2023). USAGE OF ANTIMICROBIAL ACTIVITY AGAINST E. COLI O157:H7 ISOLATED FROM LOCAL MEAT AND VEGETABLES IN ERBIL CITY. , 21(1), 114-123. doi: 10.32649/ajas.2023.179721
R. A. Jalal; Kh. E. Aziz. "USAGE OF ANTIMICROBIAL ACTIVITY AGAINST E. COLI O157:H7 ISOLATED FROM LOCAL MEAT AND VEGETABLES IN ERBIL CITY". , 21, 1, 2023, 114-123. doi: 10.32649/ajas.2023.179721
A. Jalal, R., E. Aziz, K. (2023). 'USAGE OF ANTIMICROBIAL ACTIVITY AGAINST E. COLI O157:H7 ISOLATED FROM LOCAL MEAT AND VEGETABLES IN ERBIL CITY', , 21(1), pp. 114-123. doi: 10.32649/ajas.2023.179721
A. Jalal, R., E. Aziz, K. USAGE OF ANTIMICROBIAL ACTIVITY AGAINST E. COLI O157:H7 ISOLATED FROM LOCAL MEAT AND VEGETABLES IN ERBIL CITY. , 2023; 21(1): 114-123. doi: 10.32649/ajas.2023.179721

USAGE OF ANTIMICROBIAL ACTIVITY AGAINST E. COLI O157:H7 ISOLATED FROM LOCAL MEAT AND VEGETABLES IN ERBIL CITY

Anbar Journal of Agricultural Sciences
Volume 21, Issue 1, June 2023, Pages 114-123    PDF (424.35 K)
Document Type: Research Paper
DOI: 10.32649/ajas.2023.179721
Authors
R. A. Jalal; Kh. E. Aziz
Department of Food Technology, College of Agricultural Engineering Sciences, University of Salahaddin, Erbil, Iraq
Abstract
The antimicrobial effect of Escherichia coli O157:H7 was evaluated in Erbil City's fresh vegetables and meat. A total of 250 samples were collected in Erbil City. E. coli O157:H7 was successfully extracted using the ISO-16654:2001 standard. After that, the samples were isolated for antimicrobial susceptibility for ten different antimicrobials using the disk diffusion method. Only 0.51 percent of the meat and vegetable samples tested had E. coli O157: H7. According to kanamycin and ampicillin susceptibility testing results, all of the microorganisms examined were invulnerable. Erbil City's meat and vegetables are contaminated with Escherichia coli, according to this research. Despite the disease's low incidence, the fact that it may be found in a raw food product raises questions about public health implications.
References
  1. Ajuwon, B. I., Babatunde, S. K., Kolawole, O. M., Ajiboye, A. E., and Lawal, A. H. (2021). Prevalence and antibiotic resistance of Escherichia coli O157: H7 in beef at a commercial slaughterhouse in Moro, Kwara State, Nigeria. Access Microbiology, 3(11): 1-12.
  2. Ali, D. A., Tesema, T. S., and Belachew, Y. D. (2021). Molecular detection of pathogenic Escherichia coli strains and their antibiogram associated with risk factors from diarrheic calves in Jimma Ethiopia. Scientific Reports, 11(1): 1-15.
  3. Bakalis, S., Knoerzer, K., and Fryer, P. J. (2015). Modeling food processing operations. Elsevier.
  4. Bekele, T., Zewde, G., Tefera, G., Feleke, A., and Zerom, K. (2014). Escherichia coli O157: H7 in raw meat in Addis Ababa, Ethiopia: prevalence at an abattoir and retailers and antimicrobial susceptibility. International Journal of Food Contamination, 1(1): 1-8.
  5. Campos, A. C. C., Andrade, N. L., Ferdous, M., Chlebowicz, M. A., Santos, C. C., Correal, J. C., ... and Rossen, J. W. (2018). Comprehensive molecular characterization of Escherichia coli isolates from urine samples of hospitalized patients in Rio de Janeiro, Brazil. Frontiers in Microbiology, 9: 243.
  6. (2017). Performance standards for antimicrobial susceptibility testing. Clinical and Laboratory Standards Institute Wayne, PA, 12.
  7. Dejene, H., Abunna, F., Tuffa, A. C., and Gebresenbet, G. (2022). Epidemiology and antimicrobial susceptibility pattern of E. coli O157: H7 along dairy milk supply chain in Central Ethiopia. Veterinary Medicine: Research and Reports, 13: 131-142.
  8. Geletu, U. S., Usmael, M. A., and Ibrahim, A. M. (2022). Isolation, Identification, and Susceptibility Profile of E. coli, Salmonella, and S. aureus in Dairy Farm and Their Public Health Implication in Central Ethiopia. Veterinary Medicine International, 2022: 1-10.
  9. Grace, D. (2015). Food safety in low and middle income countries. International journal of environmental research and public health, 12(9): 10490-10507.
  10. Haile, A. F., Alonso, S., Berhe, N., Bekele Atoma, T., Boyaka, P. N., and Grace, D. (2021). Escherichia coli O157: H7 in retail lettuce (Lactuca sativa) in Addis Ababa city: Magnitude of contamination and antimicrobial susceptibility pattern. Frontiers in Microbiology, 12: 694506.
  11. Humphries, R., Bobenchik, A. M., Hindler, J. A., and Schuetz, A. N. (2021). Overview of changes to the clinical and laboratory standards institute performance standards for antimicrobial susceptibility testing, M100. Journal of clinical microbiology, 59(12): e00213-21.
  12. Ibenyassine, K., Mhand, R. A., Karamoko, Y., Anajjar, B., Chouibani, M., and Ennaji, M. M. (2007). Bacterial pathogens recovered from vegetables irrigated by wastewater in Morocco. Journal of environmental health, 69(10): 47-51.
  13. Käferstein, F. K. (2003). Actions to reverse the upward curve of foodborne illness. Food control, 14(2): 101-109.
  14. Laven, R. (2006). Diagnosis of calf diarrhoea: a different perspective?. UK Vet Livestock, 11(1): 36-38.
  15. Manyi-Loh, C., Mamphweli, S., Meyer, E., and Okoh, A. (2018). Antibiotic use in agriculture and its consequential resistance in environmental sources: potential public health implications. Molecules, 23(4): 795-809.
  16. Megersa, R., Mathewos, M., and Fesseha, H. (2019). Isolation and Identification of Escherichia coli from dairy cow raw milk in Bishoftu Town, Central Ethiopia. Archives of Veterinary and Animal Sciences, 1(1).
  17. Meng, J., and Doyle, M. P. (1998). Emerging and evolving microbial foodborne pathogens. Bulletin de l'Institut Pasteur, 96(3): 151-163.
  18. Moses, A. E., Garbati, M. A., Egwu, A. O., and Ameh, E. J. (2006). Detection of E. coli 0157 and 026 serogroups in human immunodeficiency virus-infected patients with clinical manifestation of diarrhoea in Maiduguri, Nigeria. Research Journal of Medicine and Medical Sciences, 1(4): 140-145.
  19. Quinn, P. J., Markey, B. K., Leonard, F. C., Hartigan, P., Fanning, S., and Fitzpatrick, E. (2011). Veterinary microbiology and microbial disease. John Wiley and Sons.
  20. Saadi Al-Baer, A., and Hussein, A. A. (2017). Isolation and identification of Escherichia coli producing cytosine deaminase from Iraqi patients. International Journal of Advanced Research in Biological Sciences, 4(11): 1-6.
  21. Saeed, A. Y., Mazin, H., Saadi, A., and Hussein, S. O. (2013). Detection of Escherichia coli O157 in vegetables. IOSR Journal of Agriculture and Veterinary Science, 6(2): 16-18.
  22. (2017). IBM SPSS Statistics for Windows, version 25. Armonk, NY: IBM SPSS Corp.
  23. Ulukanli, Z., Çavli, P., and Tuzcu, M. (2006). Detection of Escherichia coli O157: H7 from beef doner kebabs sold in Kars. Gazi University Journal of Science, 19(2): 99-104.
  24. Wang, S., Zhang, S., Liu, Z., Liu, P., Shi, Z., Wei, J., ... and Ma, Z. (2014). Molecular characterization of enterohemorrhagic E. coli O157 isolated from animal fecal and food samples in Eastern China. The Scientific World Journal.
  25. Weinstein, M. P. (2021). Performance standards for antimicrobial susceptibility testing, Clinical and Laboratory Standards Institute.
Statistics
Article View: 1,721
PDF Download: 392


Journal Management System. Powered by iJournalPro.com