Phylogenetic Analysis of 16S rRNA Gene of Burkholderia cepacia Complex Species Isolated from Different Clinical Sources

Ibrahim, Hazim Mohammad; Alsammak, Essra Gh.

doi:10.33899/rjs.2023.180288

	Phylogenetic Analysis of 16S rRNA Gene of Burkholderia cepacia Complex Species Isolated from Different Clinical Sources
Rafidain Journal of Science
Volume 32, Issue 3, September 2023, Pages 19-30 PDF (1.02 M)
Document Type: Research Paper
DOI: 10.33899/rjs.2023.180288
Authors
Hazim Mohammad Ibrahim¹; Essra Gh. Alsammak²
¹Nineveh Health Directorate / Ministry of Health/Iraq
²Department of Biology/ College of Science/ University of Mosul
Abstract
Twenty hundred and fifty clinical samples were collected from different clinical sources for patients of both sexes for the period from October 2021 to May 2022. These samples were obtained from several hospitals in Mosul city (Al-Jumhouri Teaching Hospital, Al-Salam Teaching Hospital, Ibn Sina Teaching Hospital, Mosul specialized center for burns and surgery aesthetics and Fayda health center). Out of 250 samples, 47 samples showed no growth at a rate of 18.8%, while 203 samples at 81.2% showed bacterial growth on Maconkey agar medium. The diagnosis was confirmed by using Oxidation Fermentation Polymyxin Bacitracin Lactose Agar medium, 18 isolates were diagnosed as Bcc related and the percentage of isolates obtained reached 7.2% of the total diseased (250), which included 2.4% (6) of burns, 1.6% (4) of wounds, 1.2% (3) of urine, 1.2% (3) of diabetic foot ulcer and 0.8% (2) of sputum. Bcc isolates were characterized by the production of virulence factors, including urease enzyme, slime layer production, biofilm production, gelatinase enzyme production and hemolysin production. With percentages of (100%) 21, (100%) 21, (100%) 21 (100%), (66.7 %) 14 and (47.6%) 10, respectively. The sequencing of 16S rRNA genes were compared with the gene sequences of the references isolates in the Basic Local Alignment Search Tool (BLAST) within the NCBI database. A tree diagram was obtained in which the strains belonging to the Bcc group were distributed into five clusters A, B, C, D, and E at 97% similarity for cluster A, 97.4% similarity for cluster B, 97.8% similarity for cluster C, and at 97.6% similarity for cluster D, while cluster E included one isolate that was associated at 95% by using Mega 7 program and UPGMA method The strains HE2 and HE14 were submitted to the GenBank under the accession numbers OP218010 and OP218012, respectively. It was found that these bacteria are resistant to many antibiotics, but they showed a kind of sensitivity to the antibiotic Meropenem. The multi-drug resistance of bacteria also corresponds to their possession of efflux pumps.
Keywords
Bcc; OFPBL; 16S rRNA; BLAST

Statistics Article View: 468 PDF Download: 206