Khalil, R. (2023). Evaluation of Various In-vitro Biological Activities of Crude Quince Seed Ethanolic Extract. , 20(Supplementary Issue 1), 217-223. doi: 10.33899/iphr.2023.144158.1063
Raghad Riyadh Khalil. "Evaluation of Various In-vitro Biological Activities of Crude Quince Seed Ethanolic Extract". , 20, Supplementary Issue 1, 2023, 217-223. doi: 10.33899/iphr.2023.144158.1063
Khalil, R. (2023). 'Evaluation of Various In-vitro Biological Activities of Crude Quince Seed Ethanolic Extract', , 20(Supplementary Issue 1), pp. 217-223. doi: 10.33899/iphr.2023.144158.1063
Khalil, R. Evaluation of Various In-vitro Biological Activities of Crude Quince Seed Ethanolic Extract. , 2023; 20(Supplementary Issue 1): 217-223. doi: 10.33899/iphr.2023.144158.1063

Evaluation of Various In-vitro Biological Activities of Crude Quince Seed Ethanolic Extract

Iraqi Journal of Pharmacy
Volume 20, Supplementary Issue 1, December 2023, Pages 217-223    PDF (635.1 K)
Document Type: Research Paper
DOI: 10.33899/iphr.2023.144158.1063
Author
Raghad Riyadh Khalil*
Department of Pharmaceutical Chemistry, College of Pharmacy, University of Mosul, Mosul, Iraq
Abstract
Background: for a long time, natural bio-actives have been used to treat, cure, and prevent illnesses. Fruit seed crude extracts have been found to have various beneficial biological activities, such as anti-inflammatory, antioxidant, and antitumoral potential. Methods: this study investigated quince seed extract made using absolute ethanol as a solvent regarding flavonoid and phenolic content, as well as antioxidant, anti-inflammatory, and anticancer properties. The extract was obtained using two methods, microwave-facilitated extraction and sequential microwave-ultrasound-assisted extraction. Results: the extract from the microwave-facilitated method had higher flavonoid and phenolic content with superior antioxidant activity. Hydroxyl free radical and DPPH assays' IC50 were 121.04±1.10 and 112.98±1.02, respectively, compared to the sequential method, which had IC50 values of 193.19±0.86 and 187.23±0.95. The microwave method also displayed significantly higher inhibitory activity against inflammatory enzymes and selectivity against COX2 compared to the sequential one. However, the extracts did not exhibit any antiproliferative effect on malignant or healthy cellular lines at the applied concentrations, and they did not enhance the effectiveness of 5-FU (5-Fluorouracil) in preventing the growth of cancerous cells. However, the mutual application in general results in increasing the IC50 value of 5-FU toward the healthy cellular line (MCF-A10). Therefore, the mutual application of these extracts with 5-FU can be seen as a protective measure. Conclusion: the crude extract obtained through the microwave-facilitated method had better TPC, TFC, antioxidant, anti-inflammatory activity, and protective potential than the sequential method.
Keywords
Quince seeds; Ethanolic extract; Anti-inflammatory; Antioxidant; Antitumor
References
  1. Tonin LT, Camargo JN, Bertan AS, Almeida IV, Vicentini VE, Dusman E. Antitumoral activity, antioxidant capacity and bioactive compounds of ginger (Zingiber officinale). Acta Scientiarum. Technology. 2020;42:e45724-
  2. Singh AK, Kim JY, Lee YS. Phenolic compounds in active packaging and edible films/coatings: natural bioactive molecules and novel packaging ingredients. Molecules. 2022 Nov 3;27(21):7513.
  3. Górnaś P, Rudzińska M. Seeds recovered from industry by-products of nine fruit species with a high potential utility as a source of unconventional oil for biodiesel and cosmetic and pharmaceutical sectors. Industrial Crops and Products. 2016 May 1;83:329-38.
  4. Khalil RR, Mohammed ET, Mustafa YF. Evaluation of in vitro antioxidant and antidiabetic properties of cydonia oblonga seeds’ extracts. Journal of Medicinal and Chemical Sciences. 2022;5(6):1048-58.
  5. Al-Snafi AE. The medical importance of Cydonia oblonga-A review. IOSR Journal of Pharmacy. 2016 Jun;6(6):87-99.
  6. Afonso AF, Pereira OR, Cardoso SM. Health-promoting effects of Thymus phenolic-rich extracts: Antioxidant, anti-inflammatory and antitumoral properties. Antioxidants. 2020 Sep 1;9(9):814.
  7. Mustafa YF, Mohammed ET, Khalil RR. Antioxidant and antitumor activities of methanolic extracts obtained from Red delicious and Granny Smith apples’ seeds. Systematic Reviews in Pharmacy. 2020;11(4):570–6.
  8. Fidelis M, de Moura C, Kabbas Junior T, Pap N, Mattila P, Mäkinen S, Putnik P, Bursać Kovačević D, Tian Y, Yang B, Granato D. Fruit seeds as sources of bioactive compounds: Sustainable production of high value-added ingredients from by-products within circular economy. Molecules. 2019 Oct 25;24(21):3854.
  9. Gorgani L, Mohammadi M, Najafpour GD, Nikzad M. Sequential microwave-ultrasound-assisted extraction for isolation of piperine from black pepper (Piper nigrum L.). Food and Bioprocess Technology. 2017 Dec;10:2199-207.
  10. Amin T, Naik HR, Hussain SZ, Jabeen A, Thakur M. In-vitro antioxidant and antibacterial activities of pumpkin, quince, muskmelon and bottle gourd seeds. Journal of Food Measurement and Characterization. 2018;12(1):182–90.
  11. Mohammed ET, Khalil RR, Mustafa YF. Phytochemical analysis and antimicrobial evaluation of quince seeds’ extracts. Journal of Medicinal and Chemical Sciences. 2022;5(6):968-79.
  12. Babashpour-Asl M, Piryaei M. Free radical scavengering and phenolic compounds of peel and pulp of quince. International Journal of Horticultural Science and Technology. 2021 Jan 1;8(1):91-101.
  13. Velisdeh ZJ, Najafpour GD, Mohammadi M, Poureini F. Optimization of Sequential Microwave-Ultrasound-Assisted Extraction for Maximum Recovery of Quercetin and Total Flavonoids from Red Onion (Allium cepa L.) Skin Wastes. arXiv preprint arXiv:2104.06109. 2021 Apr 13.
  14. Khalil RR, Mustafa YF. Phytochemical, antioxidant and antitumor studies of coumarins extracted from Granny Smith apple seeds by different methods. Systematic Reviews in Pharmacy. 2020;11(2):57–63.
  15. Jan MS, Shahid M, Ahmad S, Hussain F, Ahmad A, Mahmood F, Rashid U, Ullah F, Khan N, Aasim M. Synthesis of pyrrolidine-2, 5-dione based anti-inflammatory drug: in vitro COX-2, 5-LOX inhibition and in vivo anti-inflammatory studies. Latin American Journal of Pharmacy. 2019 Jan 1;38(11):2287-94.
  16. Bajaj S, Fuloria S, Subramaniyan V, Meenakshi DU, Wakode S, Kaur A, Bansal H, Manchanda S, Kumar S, Fuloria NK. Chemical characterization and anti-inflammatory activity of phytoconstituents from Swertia alata. Plants. 2021 May 31;10(6):1109.
  17. Fernández J, Silván B, Entrialgo-Cadierno R, Villar CJ, Capasso R, Uranga JA, Lombo F, Abalo R. Antiproliferative and palliative activity of flavonoids in colorectal cancer. Biomedicine & Pharmacotherapy. 2021 Nov 1;143:112241.
  18. Zhang YJ, Gan RY, Li S, Zhou Y, Li AN, Xu DP, Li HB. Antioxidant phytochemicals for the prevention and treatment of chronic diseases. Molecules. 2015 Nov 27;20(12):21138-56.
  19. Kozłowska M, Gruczyńska E, Ścibisz I, Rudzińska M. Fatty acids and sterols composition, and antioxidant activity of oils extracted from plant seeds. Food chemistry. 2016 Dec 15;213:450-6.
  20. Mohammed ET, Mustafa YF. Coumarins from red delicious apple seeds: Extraction, phytochemical analysis, and evaluation as antimicrobial agents. Systematic Reviews in Pharmacy. 2020;11(2):64–70.
  21. Mustafa YF, Khalil RR, Mohammed ET, Bashir MK, Oglah MK. Effect of chemical modification involving phenolic hydroxyl group on the biological activity of natural coumarins. Pakistan Journal of Medical & Health Sciences. 2021;15(3):954–61.
  22. Phuyal N, Jha PK, Raturi PP, Rajbhandary S. Total phenolic, flavonoid contents, and antioxidant activities of fruit, seed, and bark extracts of Zanthoxylum armatum DC. The Scientific World Journal. 2020 Mar 16;2020.
  23. Maulana TI, Falah S, Andrianto D. Total phenolic content, total flavonoid content, and antioxidant activity of water and ethanol extract from Surian (Toona sinensis) leaves. In IOP Conference Series: Earth and Environmental Science (Vol. 299, No. 1, p. 012021). IOP Publishing.
  24. Truong DH, Nguyen DH, Ta NT, Bui AV, Do TH, Nguyen HC. Evaluation of the use of different solvents for phytochemical constituents, antioxidants, and in vitro anti-inflammatory activities of Severinia buxifolia. Journal of food quality. 2019 Jan 1;2019.
  25. Guo Q, Li F, Duan Y, Wen C, Wang W, Zhang L, Huang R, Yin Y. Oxidative stress, nutritional antioxidants and beyond. Science China Life Sciences. 2020 Jun;63:866-74.
  26. Shaida B, Singh NB, Singh K. In-vitro evaluation of anti-inflammatory and anti-microbial properties of ethanolic extract of cydonia oblonga seeds. Journal of Scientific and Industrial Research. 2020;79(1):49–52.
  27. Kalisz S, Oszmiański J, Kolniak-Ostek J, Grobelna A, Kieliszek M, Cendrowski A. Effect of a variety of polyphenols compounds and antioxidant properties of rhubarb (Rheum rhabarbarum). LWT - Food Science and Technolog. 2020 Jan 1;118:108775.
  28. Hou C, Chen L, Yang L, Ji X. An insight into anti-inflammatory effects of natural polysaccharides. International journal of biological macromolecules. 2020 Jun 15;153:248-55.
  29. Nunes CD, Barreto Arantes M, Menezes de Faria Pereira S, Leandro da Cruz L, de Souza Passos M, Pereira de Moraes L, Vieira IJ, Barros de Oliveira D. Plants as sources of anti-inflammatory agents. Molecules. 2020 Aug 15;25(16):3726.
  30. Moutabian H, Majdaeen M, Ghahramani-Asl R, Yadollahi M, Gharepapagh E, Ataei G, Falahatpour Z, Bagheri H, Farhood B. A systematic review of the therapeutic effects of resveratrol in combination with 5-fluorouracil during colorectal cancer treatment: With a special focus on the oxidant, apoptotic, and anti-inflammatory activities. Cancer cell international. 2022 Dec;22(1):1-4.
  31. Gupta D, Guliani E. Flavonoids: Molecular mechanism behind natural chemoprotective behavior-a mini review. Biointerface Research in Applied Chemistry. 2022;12(5):5983-95.
  32. Xiao Q, Zhu W, Feng W, Lee SS, Leung AW, Shen J, Gao L, Xu C. A review of resveratrol as a potent chemoprotective and synergistic agent in cancer chemotherapy. Frontiers in pharmacology. 2019 Jan 9;9:1534.
 

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