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Estimation of Gene Expression by Differential Display Technique of Potato Cultivars (Solanum Tuberosum L.) Grown in vitro Under Drought Stress | ||
| IRAQI JOURNAL OF DESERT STUDIES | ||
| Article 1, Volume 13, Issue 2, December 2023, Pages 1-9 PDF (716.35 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.36531/ijds.2023.141560.1041 | ||
| Authors | ||
| Tuqa R. Noor1; Radhi Dheyab ِِAbed* 2; Shatha A. Yousif3 | ||
| 1Seed Inspection and Certification Directorate, Ministry of Agriculture, Baghdad, Iraq | ||
| 2University of Fallujah, College of Applied Sciences | ||
| 3Agricultural Research Directorate, Science and Technology, Baghdad, Iraq | ||
| Abstract | ||
| A laboratory experiment was carried out at Genetic Engineering Department- Agricultural Research Directorate/ Ministry of Science and Technology for the period between March 2019 and May 2020. The aim was to characterize the gene expression at different rates of drought stress. Stem cuttings were cultured in MS medium which contained three concentrations of Poly Ethylene Glycol (PEG) as a stress factor (0, 0.5 and 2.5 %). Differential Display Reverse Trancrptase- DDRT was applied to study the genetic markers related with drought tolerance by using 6 randomized primers (B5, D2, E12, F12, Q16 and H9). The experiment included seven genotypes of potatoes, four of which were certificated in Iraq, namely Arnova, Burren, Provento and Riviera and the rest were brought from the International Potato Institute in Peru which are 58, 60 and 67. All primers (6 primers) gave a polymorphism, with a total of 101 bands. Maybe this indicate the existence of gene expression in plants that subjected to drought stress. The primers varied in number of bands, the lowest number of bands were in D2 which gave 15 bands while the highest number of bands (18 bands) were in B5, F12 and H9. A number of bands of all primers studied were observed in plants under control and drought stresses, indicating the possibility of these DNA fragments involved in drought tolerance. | ||
| Keywords | ||
| polymorphism; differential display; potato; gene expression; bands | ||
| References | ||
|
Al-Judy, N. & Majeed, R. (2013). Morphological, biochemical, and molecular characterization of ten rhizobial bacteria isolates. Iraqi journal of science, 54(2),280-287.
AL-Kazaz, A. (2001). Isolation and characterization of a salt- induced transcript from halophyte Spartina anglica using a modified version of DDRT- PCR. PhD Thesis. College of Science, Nankai University, China.
Alves, J, VanToai, T. & Namik, K. (1998). Differential display: a novel PCR based method for gene isolation and cloning. Revista Brasileira. de Andreea, N., Mihaela, C., Nicoleta, C. & Monica, N. (2014). In vitro response to drought tolerance for different potato varieties. Analele Universitaţii din Oradea, Fascicula Protecţia Mediului, 257-262.
Ambrosone, A., Batelli, G., Bostan, H., D’Agostino, N., Chiusano, M., Perrotta, G., Leone, A., Grillo, S. & Costa, A. (2017). Distinct gene networks drive differential response to abrupt or gradual water deficit in potato. Gene, 597,30–39.
Byun, M.O., Kwon, H.B. & Park, S.C. (2007). Recent advances in genetic engineering of potato crops for drought and saline stress tolerance. In Jenks, M.A, Hasegawa, P.M. & Jain, M. (eds.) Advances in Molecular Breeding toward Drought and Salt Tolerant Crops. Springer, Dordrecht. pp. 713-737.
Duresa, O., Mohamed, C. & Teju. E. (2018). Genetic variability, correlation and path coefficient analysis in potato genotypes for yield, yield related traits and mineral contents at Haramaya. PhD thesis, Haramaya University, Ethiopia.
Fislage, R. Barchans, M., Humboldt, Y., Wendt, M. & Oberender, H. (1997). Primer design for a prokaryotic differential display RT-PCR. Nucleic Acids Research, 25(9), 1830–1835.
Hadi, S. M., Ibrahim, K & Yousif, Sh. (2019). Differential expression for genes in response to drought and salinity in Ruta graveolens plantlets. Jordan Journal of Biological Sciences, 12(2), 203-207.
Hosseinizadeh, A., SeyedKaboli, H., Zareie, H., Akhondali, A. & Farjad, B. (2015). Impact of climate change on the severity, duration, and frequency of drought in a semi-arid agricultural basin. Geoenvironmental Disasters, 2(1),23.
Jenkins, K. & Warren R. (2015). Quantifying the impact of climate change on drought regimes using the Standardized Precipitation Index. Theoretical and Applied Climatology, 120(1),41–54.
Krishna, H.S. 2002. Developments and trends in enzyme catalysis in nonconventional media. Biotechnology Advances, 20,239-266.
Liang, P. & Pardee, A. B. (1992). Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science, 257, 967- 971.
Liang, P. (2002). A decade of differential display. Biology Techniques, 33(2), 338-346.
Murashige, T. & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiology Plantarum, 15, 473-497.
Nasiruddin, K.M., Yasmin, S., Toma, S.M. & Crescenzi, A. (2005). Screening of potato germplasm against abiotic stress and molecular characterization by Randomly Amplified Polymorphic DNA analysis. Acta Horticulture, 684,143-150.
Pieczynski, M., Wyrzykowska, A., Milanowska, K., Boguszewska-Mankowska, D., Zagdanska, B., Karlowski, W., Jarmolowski, A., & Szweykowska-Kulinska, Z. (2018) Genome wide identification of genes involved in the potato response to drought indicates functional evolutionary conservation with Arabidopsis plants. Plant Biotechnology Journal, 16(2),603–614.
Sabbah, M. (2013). Differential screening of randomly amplified cDNAs using RAPD primers in salt tolerance and sensitive wheat. Ibn Al-Haitham Journal for Pure & Applied Sciences, 26(3), 43-48.
Shahid, M, Jamal, A., Rashid, B., Aftab, B. & Husnain, T. (2012). Identification and isolation of salt stress−responsive transcripts from Gossypium arboretum L. Turkish Journal of Biology, 36,746-756.
Whitmore, A. & Whalley, W. (2009) Physical effects of soil drying on roots and crop growth. Journal of Experimental Botany, 60(10),2845–2857.
Yoshida, K, Naito, S. & Takeda, G. (1994). cDNA cloning of regeneration-specific genes in rice by differential screening of randomly amplified cDNAs using RAPD primers. Plant Cell Physiology. 35(7),1003-1009. | ||
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