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MOLECULAR CHARACTERISATION OF LUMPY SKIN DISEASE VIRUS IN CATTLE USING P32 AND RPO30 GENES IN DUHOK PROVINCE, KURDISAN REGION OF IRAQ. | ||
| Basrah Journal of Veterinary Research | ||
| Volume 19, Issue 1, 2020, Pages 247-264 PDF (820.38 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.23975/bjvetr.2020.182399 | ||
| Author | ||
| Ramadhan Ado Khanamir , Jivan Qasim Ahmed, Meqdad Saleh Ahmed, Karwan Idrees Jarjees . | ||
| Abstract | ||
| Lumpy skin disease (LSD) is a contagious viral infection of cattle caused by lumpy skin disease virus (LSDV). This infection has a huge economic impact on the cattle industry as a result of skin damage, inflammation of mammary glands and decrease milk production, reduces reproduction and even death as a consequence of secondary bacterial infection. Iraq is considered as an endemic country by OIE that continues breaking throughout different parts of the country. The aims of this research work was to detect and molecular characterization of LSDV in Duhok province for the first time through using a phylogenetic approach of P32 and RPO30 genes. The results showed that LSDV was detected and identified over using PCR and sequencing and the isolate 1 LSDV/Duhok/Kurdistan/2019, isolate 2 LSDV/Duhok/Kurdistan/2019 and isolate 3 LSDV/Duhok/Kurdistan/2019 were clustered in LSDV lineage for both P32 and RPO30 genes. The phylogenetic analysis of these isolates showed a close relationship with the previously published isolates based on P32 gene sequences, while this study is the first study to publish genetic information on RPO30 gene of the Iraqi isolates and compare them to the other publicly available data sets of other countries. To sum up, the findings of this paper could be beneficial to illustrate the spreading nature of LSDV in Iraq and mainly in the Kurdistan region of Iraq. Furthermore, offer the theoretical references for control and prevention of LSDV infections in future. | ||
| Keywords | ||
| P32 gene; RPO30 gene; Phylogenetic analysis | ||
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