Isolation of Pseudomonas Aeruginosa and Studying their Resistance and Pyocyanin Production.

Hameed, Farah Ali

doi:10.32894/kujss.2024.145615.1132

	Isolation of Pseudomonas Aeruginosa and Studying their Resistance and Pyocyanin Production.
Kirkuk Journal of Science
Article 3, Volume 19, Issue 1, March 2024, Pages 24-31 PDF (764.87 K)
Document Type: Research Paper
DOI: 10.32894/kujss.2024.145615.1132
Author
Farah Ali Hameed^*
Department of Emergency Medicine and First Aids Techniques, Institute of Medical Technology, Middle Technical University, Baghdad, Iraq.
Abstract
The study included isolating and diagnosing Pseudomonas Aerugi- nosa bacteria from different clinical samples from hospitals and health centers: 10 wound samples, 15 burn samples, 12 ear samples (otitis media), 11 urine samples and 9 sputum samples. These samples were diagnosed using cultural and biochemical features and confirmed by using the API 20E system test. The results showed that the isolation rate of Pseudomonas Aeruginosa bacteria from burns was (26.3%) and from ears was (21%). The resistance of Pseudomonas Aeruginosa isolates to 6 types of antibiotics was tested. The highest resistance rate (29.8%) was observed against Cefazoline and Carbenicillin, followed by resistance to Cefotaxime (17.5%), then to Gentamicin (14%), followed by Ciprofloxacin and Amikacin, (5.2%) and (3.5%), respectively. The results demonstrated that 18(31.5%) of Pseudomonas Aeruginosa isolates produced the pyocyanin pigment on Nutrient agar media and 9(15.7%) of them produced the pigment on MacConkey agar, while 15(26.3%) isolates produced the pigment on Blood agar. The burn isolates were shown to produce the highest pyocyanin pigments, and nutrient agar was the highest media that produced pyocyanin.
Keywords
Pseudomonas aeruginosa; clinical samples; resistance; pyocyanin pigment

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