The Effects Of Eexogenous Free Radicals And Ultraviolet CRadiation On The Cytoskeleton Membrane Protein Of HumanRed Blood Cells.

M. Al-Deen, Suad; Anwer Raoof, May; W. Abdul-Lateef, Zainab

	The Effects Of Eexogenous Free Radicals And Ultraviolet CRadiation On The Cytoskeleton Membrane Protein Of HumanRed Blood Cells.
Diyala Journal For Pure Science
Article 1, Volume 7, Issue 2, 2011, Pages 156-165
Authors
Suad M. Al-Deen; May Anwer Raoof; Zainab W. Abdul-Lateef
Abstract
Objectives: This study aimed to explore the effect of exogenous free radicals and ultravioletC (UVC) radiation on the protein and peptides of cytoskeleton membranes isolated fromhuman red blood cellsMethods: Cytoskeleton membranes protein of human red blood cells were isolated andincubated with different concentrations of exogenous free radicals notably peroxynitrite(ONOO-), hydrogen peroxide (H2O2) or hydrochlorate (HOCl) for 5 minutes. In another seriesof experiments, the cytoskeleton membranes protein were subjected to UVC radiation (λ 254nm) up to 30 minutes. The total protein was measured spectrophotometrically by Bradfordmethod and the peptides levels were also measured spectrophotometrically.Results : All the exogenous free radicals completely destroyed the protein of cytoskeletonmembranes of human red cells and this effect is associated with increase peptides level in adose dependent manner with ONOO- (65.86-329.34 μM), H2O2 (14.7-73.52 μM), but not withHOCl (9.52-47.62 μM). Variable damaging effects of UVC radiation were observed on bothtotal protein and peptides. The effect of UVC radiation was not related to the exposure period.Conclusions : Protein cytoskeleton membrane of human red cells is directly damaged byexogenous free radicals and while the effect of UVC radiation is resulted from both directradiation effect, and indirect via releasing free radicals in aqueous solution.

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