In Vitro Evaluation of The Anti-Proliferative Effect of Quercus Infectoria Crude Extracts on MCF-7 Cell Line

Mustafa, Fatima Wasfi; Al-jalily, Omar Riadh; Alasady, Asaad Abdulwahid

doi:10.33899/mmed.2024.146461.1251

	In Vitro Evaluation of The Anti-Proliferative Effect of Quercus Infectoria Crude Extracts on MCF-7 Cell Line
Annals of the College of Medicine, Mosul
Volume 46, Issue 1, June 2024, Pages 36-42 PDF (1.32 M)
Document Type: Research Paper
DOI: 10.33899/mmed.2024.146461.1251
Authors
Fatima Wasfi Mustafa^* ¹; Omar Riadh Al-jalily¹; Asaad Abdulwahid Alasady²
¹Department of Anatomy, College of Medicine, University of Mosul ,Mosul
²Dept. of Anatomy, College of Medicine, University of Duhok
Abstract
Breast tumor ranks as the most prevalent cancer between female globally, spinning both developed and developing nations. In 2018, Breast cancer came in second place among the most common cancers, while came in the fifth place among the most common causes of cancer death. Chemotherapy, the primary method in cancer treatment, utilizing specific medications to manage the spread of cancer cells. However, these drugs have been known to exhibit adverse effects on non-cancerous cells. Consequently, there is a pressing need to create new anticancer medications that selectively target cancer cells. In this context, natural products from plants are anticipated to yield potential candidates for the development of targeted anticancer drugs. Therefore, this study targeted to appraise the anti-proliferative effect of Gall Oak (Quercus infectoria Oliv.) as an alternative medicine on human breast MCF-7 cancer cell line. Oak galls were extracted using three solvents (80% Ethanol, Ethyl Acetate and Petroleum Ether 40° - 60°), which differ in polarity, using SER 148 Solvent Extractor. Colorimetric detection was conducted to determine the phytochemical constituents of the extracts. The cytotoxic action of the extracts using Crystal Violet stain on MCF-7 cell line was evaluated. Eight concentrations of each extract were prepared (0.78, 1.56, 3.125, 6.25, 12.5, 25, 50 and 100 µg/ml). With three replicates for each concentration, optical density (OD) of cell growth was read by a Multi-detection plate reader at a transmitting wavelength of 492 nm. The results of the study indicate that the best inhibitory activity of the oak galls petroleum ether extract on MCF-7 cell line was found at 25 µg/ml concentration after 48 hours of treatment.
Keywords
Quercus infectoria; MCF-7; cytotoxicity

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