Antibiofilm Activity of Mersacidin produced by Bacillus sp.-AE Against Acinetobacter baumannii and Acinetobacter junii isolated from Clinical Specimens.

Al-Najim, Alaa N. Mahmood; Alsammak, Essra Ghanim

doi:10.33899/rjs.2024.183433

	Antibiofilm Activity of Mersacidin produced by Bacillus sp.-AE Against Acinetobacter baumannii and Acinetobacter junii isolated from Clinical Specimens.
Rafidain Journal of Science
Volume 33, Issue 2, June 2024, Pages 21-39 PDF (1.22 M)
Document Type: Research Paper
DOI: 10.33899/rjs.2024.183433
Authors
Alaa N. Mahmood Al-Najim^* ¹; Essra Ghanim Alsammak^* ²
¹Department of Biology / College of Sciences / University of Mosul
²Dept. of Biology, College of Science, Mosul University, Mosul, IRAQ
Abstract
One hundred and sixty clinical Specimens were collected, of which 120 isolates belonging to genus Acinetobacter spp, for 75%, and the highest percentage of burns was 85.7%, wounds 75.6%, diabetic foot ulcers 75%, tonsil smears 66.6%, urine from cases of urinary tract infection and kidney failure 50%, and bone marrow fluid spiny C.S.F by 25%. from various Mosul hospitals. Thirteen isolates belonging to the two species Acinetobacter baumannii-AE and Acinetobacter junii-AE were selected based on morphological traits and biochemical tests. The phylogenetic relationship based on 16SrRNA The Acinetobacter species clustered in 3 clusters: A: include 8 strains A. baumannii-AE at 98.05%, B: include 2 strains A. junii-AE at 98.2% and C: include 3 strains A. junii-AE at 98.75% were obtained using (Mega7) program and method Clustering depended on (UPGMA). The results showed that the genus Acinetobacter produced a biofilm at 53.8% strongly, while the average production on CRA medium was 46.1%, while the results on MTP 53.8% strong production of the mucous layer in abundance with dark colonies and 46.1% medium producing biofilm with brown colonies, the minimum inhibitory concentration of Mersacidin extracted from strain Bacillus sp.-AE against pathogenic isolates A.baumannii and A.junii ranged between (625-2500) μg/ml, while minimum sub MIC ranged between (312.5-1250) µg/mL, and the strain Bacillus sp.-AE Mersacidin-producing bacteriocins have the highest anti-biofilm effect than A.baumannii and A.junii strains. is a strong biofilm-forming.
Keywords
Acinetobacter; Mersacidin; Biofilm; Bacillus sp

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