Hamad, M., Abdulgafor, A. (2023). Molecular Detection of Pseudomonas aeruginosa in Feline Otitis Externa. , 22(Special issue: Proceedings for the Scientific Conference of the College of Veterinary Medicine, University of Al-Qadisiyah (7th National/2nd International-2023)), 27-33. doi: 10.29079/qjvms.2023.183454
Maryam Abubakr Hamad; Ayman Barzan Abdulgafor. "Molecular Detection of Pseudomonas aeruginosa in Feline Otitis Externa". , 22, Special issue: Proceedings for the Scientific Conference of the College of Veterinary Medicine, University of Al-Qadisiyah (7th National/2nd International-2023), 2023, 27-33. doi: 10.29079/qjvms.2023.183454
Hamad, M., Abdulgafor, A. (2023). 'Molecular Detection of Pseudomonas aeruginosa in Feline Otitis Externa', , 22(Special issue: Proceedings for the Scientific Conference of the College of Veterinary Medicine, University of Al-Qadisiyah (7th National/2nd International-2023)), pp. 27-33. doi: 10.29079/qjvms.2023.183454
Hamad, M., Abdulgafor, A. Molecular Detection of Pseudomonas aeruginosa in Feline Otitis Externa. , 2023; 22(Special issue: Proceedings for the Scientific Conference of the College of Veterinary Medicine, University of Al-Qadisiyah (7th National/2nd International-2023)): 27-33. doi: 10.29079/qjvms.2023.183454

Molecular Detection of Pseudomonas aeruginosa in Feline Otitis Externa

Al-Qadisiyah Journal of Veterinary Medicine Sciences
Volume 22, Special issue: Proceedings for the Scientific Conference of the College of Veterinary Medicine, University of Al-Qadisiyah (7th National/2nd International-2023), December 2023, Pages 27-33    PDF (433.97 K)
Document Type: Research Paper
DOI: 10.29079/qjvms.2023.183454
Authors
Maryam Abubakr Hamad* ; Ayman Barzan Abdulgafor
Department of Internal and Preventive Veterinary Medicine, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq.
Abstract
Feline otitis externa is a common infection in small animals and the treatment can be challenging especially when the bacteria is multidrug resistant such as pseudomonas aeruginosa. For this reason, the present study aimed to investigate the prevalence of pseudomonas aeruginosa as a causative agent for feline otitis externa. The PCR assay was applied for the detection of P. aeruginosa from eight feline otitis externa cases based on the amplification of the 16S rRNA gene. In addition, the diagnosis depended on sequencing of the PCR products, and gene analysis was done to matched the global isolates. Present study revealed isolation of P. aeruginosa from all collected samples, and sequencing of this gene showed 99% compatibility with global results. Pseudomonas aeruginosa is an important cause of infection in feline otitis externa and the detection of this bacteria using PCR and sequence was accurate and gave a satisfactory result.
Keywords
Pseudomonas aeruginosa; feline otitis externa; PCR; sequencing; 16S rRNA
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