Al_Hassani, Z., Al-Gnami, S. (2023). Effect of Nano-Piperine and Vitamin C in Antioxidant Status in Dexamethasone Treated Male Rats. , 22(Special issue: Proceedings for the Scientific Conference of the College of Veterinary Medicine, University of Al-Qadisiyah (7th National/2nd International-2023)), 34-41. doi: 10.29079/qjvms.2023.183455
Zainab Sa'ad Al_Hassani; Sa'adeya Ali Al-Gnami. "Effect of Nano-Piperine and Vitamin C in Antioxidant Status in Dexamethasone Treated Male Rats". , 22, Special issue: Proceedings for the Scientific Conference of the College of Veterinary Medicine, University of Al-Qadisiyah (7th National/2nd International-2023), 2023, 34-41. doi: 10.29079/qjvms.2023.183455
Al_Hassani, Z., Al-Gnami, S. (2023). 'Effect of Nano-Piperine and Vitamin C in Antioxidant Status in Dexamethasone Treated Male Rats', , 22(Special issue: Proceedings for the Scientific Conference of the College of Veterinary Medicine, University of Al-Qadisiyah (7th National/2nd International-2023)), pp. 34-41. doi: 10.29079/qjvms.2023.183455
Al_Hassani, Z., Al-Gnami, S. Effect of Nano-Piperine and Vitamin C in Antioxidant Status in Dexamethasone Treated Male Rats. , 2023; 22(Special issue: Proceedings for the Scientific Conference of the College of Veterinary Medicine, University of Al-Qadisiyah (7th National/2nd International-2023)): 34-41. doi: 10.29079/qjvms.2023.183455

Effect of Nano-Piperine and Vitamin C in Antioxidant Status in Dexamethasone Treated Male Rats

Al-Qadisiyah Journal of Veterinary Medicine Sciences
Volume 22, Special issue: Proceedings for the Scientific Conference of the College of Veterinary Medicine, University of Al-Qadisiyah (7th National/2nd International-2023), December 2023, Pages 34-41    PDF (312.74 K)
Document Type: Research Paper
DOI: 10.29079/qjvms.2023.183455
Authors
Zainab Sa'ad Al_Hassani; Sa'adeya Ali Al-Gnami*
Department of Physiology and Pharmacology, College of Veterinary Medicine, University of AL-Qadisiyah, Iraq
Abstract
The study was designed to investigate whether nano-piprine and vitamin C (Vit. C) have antioxidant activity and/or it act through induction of the endogenous antioxidants. The study involved 48 male Wister rats (90 days old, 150±10g weight) randomly assigned to 6 groups (8 per group) and administered orally for 30 days. T1 was given 15 mg/kg B.W. nano-piperine, T2 was given 25 mg/kg B.W. vitamin C, T3 was given 2mg/kg B.W. dexamethasone, T4 was given both at the same time, and T5 was given both at the same time. Malondialdehyde (MDA) concentration and activities of superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH).  were measured in blood serum. The study found a substantial rise (p<0.05) in MDA levels in the T3 group given dexamethasone compared to the control group. Significant reduction (p<0.05) in MDA levels was seen in T4 and T5 groups compared to T3. Additionally, MDA levels increased significantly (p<0.05) in T1 and reduced in T2 compared to the control group. The T1 and T2 groups showed a substantial increase (p<0.05) in SOD, CAT, and GSH compared to the control group. T3 had the lowest SOD, CAT, and GSH and other experimental groups. Additionally, T4 and T5 showed a substantial increase (p<0.05) in these parameters compared to T3. It can be concluded that nano-piprine and Vit. C has antioxidant activity and plays a great role in reducing free radicals and increasing endogenous antioxidants.  
Keywords
Dexamethasone; Antioxidants; Nano-Piperine; Vit.C
References
  1. Fang YZ, Yang S, Wu G. Free radicals, antioxidants, and nutrition. Nutrition. 2002;18:872-879.https://doi.org/10.1016/S0899-9007(02)00916-4
  2. Poljsak B, Milisav I. The Neglected Significance of "Antioxidative Stress". Oxid Med Cell Longev. 2012;2012:480895.https://doi.org/10.1155/2012/480895
  3. Chovanová K, Böhmer M, Poljovka A, Budiš J, Harichová J, Szemeš T, Zámocký M. Parallel Molecular Evolution of Catalases and Superoxide Dismutases-Focus on Thermophilic Fungal Genomes. Antioxidants. 2020;9:1047.https://doi.org/10.3390/antiox9111047
  4. Vijayakumar R, et al. Antioxidant efficacy of black pepper (Piper nigrum L.) and piperine in rats with high-fat-diet-induced oxidative stress. Redox Report. 2004;9:105-108.https://doi.org/10.1179/135100004225004742
  5. Ahmed AE, Alshehri A, Al-Kahtani MA, Elbehairi SEI, Alshehri MA, Shati AA, Alfaifi MY, Al-Doais AA, Taha R, Morsy K, El-Mansi AA. Vitamin E and selenium administration synergistically mitigates ivermectin and doramectin-induced testicular dysfunction in male Wistar albino rats. Biomed Pharmacother. 2020;124:109841.https://doi.org/10.1016/j.biopha.2020.109841
  6. Meghwal M, Goswami T. Piper nigrum and piperine: An update. Phytother Res. 2013;27(8):1121-1130.https://doi.org/10.1002/ptr.4972
  7. Patel S, et al. Influence of co-administration of piperine on pharmacokinetic profile of gatifloxacin in layer birds. Global Veterinaria. 2011;7:427-32.
  8. Ismail OO, Ridwan IB, Olaiya OG, Abdulbasit A, Rukayat JO, Roehan FO, Abass SA. Effects of ascorbic acid on immunosuppressive drug-azathioprine-induced alteration in the liver and kidney of adult waster rats [Rattus norvegicus]. Eur J Exp Biol. 2013;3(2):371-378.
  9. Razzaq SA, Jaber IJ, Kadhim SA, Abbas AA. Pharmacological effects of dexamethasone in rats. Indian J Forensic Med Toxicol. 2020;14(3).
  10. Joda M. The Progressive Statistical Analysis by Using SPSS. First Ed. Wals House Editions, Amman Jordan; 2008.
  11. Nguelefack-Mbuyo EP, Peyembouo FP, Fofié CK, Nguelefack TB. Dose-dependent and time-dependent metabolic, hemodynamic, and redox disturbances in dexamethasone-treated Wistar rats. J Basic Clin Physiol Pharmacol. 2021.https://doi.org/10.1515/jbcpp-2020-0365
  12. Mahmoud HS, Almallah AA, Gad EL-Hak HN, Aldayel TS, Abdelrazek HMA, Khaled HE. The effect of dietary supplementation with Nigella sativa (black seeds) mediates immunological function in male Wistar rats. Sci Rep. 2021;11:7542.https://doi.org/10.1038/s41598-021-86721-1
  13. Alli J, Kehinde A, Kosoko A, Ademowo O, Adeolu A. Oxidative Stress and Reduced Vitamins C and E Levels Are Associated with Multi-Drug Resistant Tuberculosis. J Tuberc Res. 2014;2:52-58.https://doi.org/10.4236/jtr.2014.21006
  14. Adeyemi OS, Afolabi LB, Rotimi DE, Atanu FO, Ofume DS, Ogunleye T, Oluwalana DO, Oluwole TP, Youssef A, Mostafa-Hedeab G, Batiha GE-S. Differential Immunomodulatory Potential of Silver Nanoparticles and Effect on the Kynurenine Pathway in Male Wistar Rats. J Nanomater. 2022;2022:e9883142.https://doi.org/10.1155/2022/9883142
  15. Salihu M, Batiha GE-S, Kasozi KI, Zouganelis GD, Sharkawi SMZ, Ahmed EI, Usman IM, Nalugo H, Ochieng JJ, Ssengendo I, Okeniran OS, Pius T, Kimanje KR, Kegoye ES, Kenganzi R, Ssempijja F. Crinum jagus (J. Thomps. Dandy): Antioxidant and protective properties as a medicinal plant on toluene-induced oxidative stress damages in liver and kidney of rats. Toxicol Rep. 2022;9:699-712.https://doi.org/10.1016/j.toxrep.2022.03.026
  16. Mrityunjaya M, Pavithra V, Neelam R, Janhavi P, Halami PM, Ravindra PV. Immune-Boosting, Antioxidant and Anti-inflammatory Food Supplements Targeting Pathogenesis of COVID-19. Front Immunol. 2020;11:570122.https://doi.org/10.3389/fimmu.2020.570122
  17. Reda FM, Alagawany M, Mahmoud HK, Mahgoub SA, Elnesr SS. Use of red pepper oil in quail diets and its effect on performance, carcass measurements, intestinal microbiota, antioxidant indices, immunity and blood constituents. Animal. 2020;14(5):1025-1033.https://doi.org/10.1017/S1751731119002891
  18. Safaeian L, Zabolian H. Antioxidant Effects of Bovine Lactoferrin on Dexamethasone-Induced Hypertension in Rat. ISRN Pharmacol. 2014;2014:943523.https://doi.org/10.1155/2014/943523
  19. Mobarez E, Azoz H, Alkalamawy N, Nada A-F. Evaluation the Effectiveness of Green Zinc Oxide Nanoparticles on The Anti-Inflammatory Effect of Dexamethasone and Its Side Effects in Rats. SVU-Int J Vet Sci. 2018;1:25-54.https://doi.org/10.21608/svu.2018.17924
  20. Brusini R, Varna M, Couvreur P. Advanced nanomedicines for the treatment of inflammatory diseases. Adv Drug Deliv Rev. 2020;157:161-178.https://doi.org/10.1016/j.addr.2020.07.010
Statistics
Article View: 342
PDF Download: 71


Journal Management System. Powered by iJournalPro.com