Serological study of infectious bronchitis virus in vaccinated broiler chickens

AL-naimey, Zahraa A.; AL-hbiti, Thanoon Y.

doi:10.29079/qjvms.2023.183461

	Serological study of infectious bronchitis virus in vaccinated broiler chickens
Al-Qadisiyah Journal of Veterinary Medicine Sciences
Volume 22, Special issue: Proceedings for the Scientific Conference of the College of Veterinary Medicine, University of Al-Qadisiyah (7th National/2nd International-2023), December 2023, Pages 80-89 PDF (423.8 K)
Document Type: Research Paper
DOI: 10.29079/qjvms.2023.183461
Authors
Zahraa A. AL-naimey^* ; Thanoon y. AL-hbiti
Department of Pathology and Poultry Diseases, College of Veterinary Medicine, University of Mosul, Nineveh, Iraq
Abstract
Gammacoronavirus, which causes avian infectious bronchitis (IB), is characterized by respiratory and urogenital disease and causes substantial financial loss in both layers and broiler chickens. In order to determine the prevalence of antibodies against the infectious bronchitis virus (IBV) in immunized birds that showed respiratory signs as well as to evaluate the pathological changes in the trachea and lung, this preliminary study was carried out. The current work is designed to evaluate the presence of humoral immunity against IBV among six vaccinated broiler flocks in Nineveh governorate suffering from respiratory signs. At the same time, clinical signs, necropsy lesions, and histopathological findings were studied. Six pooled tracheal (T) samples and six pooled kidney (K) samples from six flocks were collected for rapid testing. On the other hand, 138 serum samples were collected to investigate the level of IBV antibodies using the indirect ELISA® test. The results of the IBV antigen rapid detection test from suspected field samples revealed that 83.3% of tracheal samples were positive, while positive kidney samples showed 66.6%. With respect to the ELISA results, they show the highest titer of 12910 and the lowest titer of 6379, with a titer mean of 9471.5. Considering the overall picture of the health status, all flocks display pathological changes. This study demonstrates that the detection of Ab titers against IBV can be successfully used for the early and rapid screening of IBV in vaccinated or infected flocks. However, due to a lack of vaccine specificity against endemic strains of IBV in Iraq, IBV infection has persisted as a challenge on chicken farms.
Keywords
Infectious bronchitis; Broilers; Rapid flow test; ELISA; Histopathology

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