Saeed, A., Abdulwahid, M. (2023). Investigation the effects of green synthesized ZnO nanoparticles on the viability of L929 fibroblasts using MTT assay. , 22(Special issue: Proceedings for the Scientific Conference of the College of Veterinary Medicine, University of Al-Qadisiyah (7th National/2nd International-2023)), 119-125. doi: 10.29079/qjvms.2023.183465
Asseel Abdulridha Saeed; Mushtaq Talib Abdulwahid. "Investigation the effects of green synthesized ZnO nanoparticles on the viability of L929 fibroblasts using MTT assay". , 22, Special issue: Proceedings for the Scientific Conference of the College of Veterinary Medicine, University of Al-Qadisiyah (7th National/2nd International-2023), 2023, 119-125. doi: 10.29079/qjvms.2023.183465
Saeed, A., Abdulwahid, M. (2023). 'Investigation the effects of green synthesized ZnO nanoparticles on the viability of L929 fibroblasts using MTT assay', , 22(Special issue: Proceedings for the Scientific Conference of the College of Veterinary Medicine, University of Al-Qadisiyah (7th National/2nd International-2023)), pp. 119-125. doi: 10.29079/qjvms.2023.183465
Saeed, A., Abdulwahid, M. Investigation the effects of green synthesized ZnO nanoparticles on the viability of L929 fibroblasts using MTT assay. , 2023; 22(Special issue: Proceedings for the Scientific Conference of the College of Veterinary Medicine, University of Al-Qadisiyah (7th National/2nd International-2023)): 119-125. doi: 10.29079/qjvms.2023.183465

Investigation the effects of green synthesized ZnO nanoparticles on the viability of L929 fibroblasts using MTT assay

Al-Qadisiyah Journal of Veterinary Medicine Sciences
Volume 22, Special issue: Proceedings for the Scientific Conference of the College of Veterinary Medicine, University of Al-Qadisiyah (7th National/2nd International-2023), December 2023, Pages 119-125    PDF (291.94 K)
Document Type: Research Paper
DOI: 10.29079/qjvms.2023.183465
Authors
Asseel Abdulridha Saeed* 1; Mushtaq Talib Abdulwahid2
1College of Veterinary Medicine, University of Al-Qadisiyah, Al-Diwaniyah City, Iraq
2College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq
Abstract
Zinc oxide nanoparticles (ZnONPs) were prepared by eco-friendly method   using the alcoholic extract of Ginger(  Zingiber officinale) ,   this study conducted to evaluate the cytotoxic effect of  Zinc oxide nanoparticles on  viability of    L929  fibroblast cell line  for 72 hours   and  incubation with (1, 2, 4, 8, and 16 mg/ml) of GnZnO NPs , Several methods were used to characterize the biosynthesized GnZnONPs. including "UV-Visible Spectroscopy," Field Emission Scanning Electron Microscope ("FESEM"), " Infrared Spectroscopy (FTIR)" and X-Ray Diffraction (XRD) spectrum.  the MTT assay was used  to determine the   cytotoxicity  effect  of Zinc oxide nanoparticles (ZnONPs)  on  the,  cell line  viability The results showed that there was no toxic effect of the synthesis GnZnONPs with an increase in concentration from (1-8) mg/ml after 72 hours of incubation at 37°C.  The findings of this study, there was a time and dose-dependent reduction in cell viability in treated cell lines after exposure to GnZnO-NPs for 3 days, with no adverse effect on fibroblast cells till 8 mg/ml.
Keywords
ZnONPs; Zingiber officinale; MTT assay; L929 fibroblast
References
  1. Abbas M, Buntinx M, Deferme W, Peeters R. Biopolymer/ZnO nanocomposites for packaging applications: a review of gas barrier and mechanical properties. Nanomaterials. 2019;9(10).https://doi.org/10.3390/nano9101494
  2. Kim IK, Viswanathan G, Kasi S, Thanakkasaranee S, Sadeghi J. ZnO nanostructures in active antibacterial food packaging: preparation methods, antimicrobial mechanisms, safety issues, future prospects, and challenges. Food Rev Int. 2020;38(4):537-65.https://doi.org/10.1080/87559129.2020.1737709
  3. Sirelkhatim A, Mahmud S, Seeni A, Kaus NH, Ann LC, Bakhori SK, Hasan H, Mohamad D. Review on zinc oxide nanoparticles: antibacterial activity and toxicity mechanism. Nano-Micro Lett. 2015;7:219-42.https://doi.org/10.1007/s40820-015-0040-x
  4. Nicole J, Binata R, Koodali TR, Adhar CM. Antibacterial activity of ZnO nanoparticle suspensions on a broad spectrum of microorganisms. FEMS Microbiol Lett. 2017;71-76.
  5. Pinho AR, Rebelo S, Pereira ML. The impact of zinc oxide nanoparticles on male (In)fertility. Materials. 2020;13:849.https://doi.org/10.3390/ma13040849
  6. Peters R, Puck B, Stefan W, Hans M, Hans B, Karin A, Hubert RV, Amenta M. Inventory of Nanotechnology applications in the agricultural, feed and food sector. EFSA J. 2014;11:7-12.https://doi.org/10.2903/sp.efsa.2014.EN-621
  7. Prashanth GK, Prashanth PA, Utpal B, Manoj G, Nagabhushana BM, Ananda S, Krishnaiah GM, Sathyananda HM. In vitro antibacterial and cytotoxicity studies of ZnO nanopowders prepared by combustion assisted facile green synthesis. Karbala Int J Mod Sci. 2015;1-67.https://doi.org/10.1016/j.kijoms.2015.10.007
  8. Prasad R. Synthesis of silver nanoparticles in photosynthetic plants. J Nanotechnol. 2014.https://doi.org/10.1155/2014/963961
  9. Khitam SS, Alhtheal ED, Azhar JB. Effect of Zinc Oxide nanoparticles preparation from Zinc Sulphate (ZnSo4) against gram negative or gram positive microorganisms in vitro. Iraqi J Vet Med. 2018;42(1):18-22.https://doi.org/10.30539/iraqijvm.v42i1.25
  10. Janak AC, Sailatha E, Gunasekaran A. Synthesis, characteristics and antimicrobial activity of ZnO nanoparticles. Spectrochim Acta Part A Mol Biomol Spectrosc. 2015;144:17-22.https://doi.org/10.1016/j.saa.2015.02.041
  11. Lim SW, Loh HS, Ting KN, Bradshaw TD, Allaudin ZN. Reduction of MTT to Purple Formazan by Vitamin E Isomers in the Absence of Cells. Trop Life Sci Res. 2015;26(1):111-20.
  12. Benov L. Improved Formazan Dissolution for Bacterial MTT Assay. Microbiol Spectr. 2021;9(3).https://doi.org/10.1128/spectrum.01637-21
  13. Ni YH, Wei XW, Hong JM, Ye Y. Hydrothermal preparation and optical properties of ZnO nanorods. Mater Sci Eng B. 2005;121:42.https://doi.org/10.1016/j.mseb.2005.02.065
  14. Kalpana VN, Rajeswari VD. A review on green synthesis, biomedical applications, and toxicity studies of ZnO NPs. Bioinorg Chem Appl. 2018;2018:3569758.https://doi.org/10.1155/2018/3569758
  15. Wang H, Li C, Zhao H, Li R, Liu J. Synthesis, characterization, and electrical conductivity of ZnO with different morphologies. Powder Technol. 2013;239:266-71.https://doi.org/10.1016/j.powtec.2012.12.045
  16. Ba-Abbad MM, Kadhum AA, Mohamad AB, Takriff MS, Sopian S. Visible light photocatalytic activity of Fe3+-doped ZnO nanoparticle prepared via sol-gel technique. Chemosphere. 2013;91:1604-11.https://doi.org/10.1016/j.chemosphere.2012.12.055
  17. Kumar P, Mahajan PK, Kaur R, Gautam S. Nanotechnology and its challenges in the food sector: a review. Mater Today Chem. 2020;17:100-332.https://doi.org/10.1016/j.mtchem.2020.100332
  18. Lozano T, Rey M, Rojas EM, Moya S, Fleddermann J, Estrela-Lopis I, Donath E, Wang B, Mao Z, Gao C. Cytotoxicity effects of metal oxide nanoparticles in human tumor cell lines. J Phys Conf Ser. 2011;304:012046.https://doi.org/10.1088/1742-6596/304/1/012046
  19. Punnoose K, Dodge JW, Rasmussen J, Chess D, Wingett C, Anders C. Cytotoxicity of ZnO Nanoparticles Can Be Tailored by Modifying Their Surface Structure: A Green Chemistry Approach for Safer Nanomaterials. ACS Sustain Chem Eng. 2014;2(7):1666-73.https://doi.org/10.1021/sc500140x
  20. Singh A, Singh NB, Afzal S, Singh T, Hussain I. Zinc oxide nanoparticles: a review of their biological synthesis, antimicrobial activity, uptake, translocation and biotransformation in plants. J Mater Sci. 2018;53:185-201.https://doi.org/10.1007/s10853-017-1544-1
  21. Górniak AK, Katarzyna R, Fernanda M, Paweł P, Bogusław B. Comparison Study of Cytotoxicity of Bare and Functionalized Zinc Oxide Nanoparticles. Int J Mol Sci. 2021;22(17):9529.https://doi.org/10.3390/ijms22179529
  22. Heim J, Felder E, Tahir MN, Kaltbeitzel A, Heinrich UR, Brochhausen C, Mailander V, Tremel W, Brieger J. Genotoxic effects of zinc oxide nanoparticles. Nanoscale. 2015;7:8931-8938.https://doi.org/10.1039/C5NR01167A
  23. Sayes CM, Reed KL, Warheit DB. Assessing toxicity of fine and nanoparticles: comparing in vitro measurements to in vivo pulmonary toxicity profiles. Toxicol Sci. 2007;97(1):163-180.https://doi.org/10.1093/toxsci/kfm018
  24. Rahman HS, Rasedee AA, Abdul B. "Zerumbone loaded nanostructured lipid carrier induces G2/M cell cycle arrest and apoptosis via mitochondrial pathway in a human lymphoblastic leukaemia cell line." Int J Nanomedicine. 2014;9:527-538.https://doi.org/10.2147/IJN.S54346
  25. Sathyabama S, Sankaranarayanan S. An In-vitro biosynthesis of zinc oxide nanoparticles using rich flavonoid extract from the petals of Delonix regia and evaluation of their antioxidant and anticancer properties. Int J Pharm Phytochem Res. 2015;7(5):1112-1119.
  26. Orazizadeh M, Khodadadi A, Bayati V, Saremy S, Farasat M, Khorsandi L. In vitro toxic effects of zinc oxide nanoparticles on rat adipose tissue-derived mesenchymal stem cells. Cell J. 2015;17(3):412-421.
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