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Study of gene expression of bacteria causing conjunctivitis | ||
| Journal of University of Anbar for Pure Science | ||
| Volume 18, Issue 1, June 2024, Pages 124-130 PDF (644.19 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.37652/juaps.2024.145798.1173 | ||
| Authors | ||
| hala Qais al halboosi* 1; Hanaa Abdellatif Yassin2; Mohammed Abbas Jasim2 | ||
| 1Department of Biology, College of Education for Women, University of Anbar, Ramadi Iraq | ||
| 2Department of Biology, College of Education for Women, University of Anbar, Ramadi, Iraq | ||
| Abstract | ||
| One hundred samples of patients with conjunctivitis who visited the Fallujah Teaching Hospital's eye consultation were included in the study. For both genders, the age range covered was 9 months to 77 years. The study found that 87% of the samples were positive for bacterial growth, while 13% were negative for it. 56.32% of them were for males and 43.67% for females. A variety of bacteria were isolated, including Staphylococcus aureus, Staph-epidermidis, and E. coli. The study demonstrates that the minimum inhibitory concentration (MIC) of each black tea aqueous extract and vancomycin was 12.5, which gives 100% inhibition. The inhibition effects of synergistic black tea extract and vancomycin were at concentrations of 6.2 giving 100% inhibition. Also, black tea extract has a beneficial effect on the gene expression of the 16S rRNA gene of E. coli bacteria in cooperation with untreated samples, which is down-regulation. Furthermore, the black tea extract affected all types of Staph-epidermidis bacteria by increasing their gene expression (upregulation) in cooperation with the untreated sample | ||
| Keywords | ||
| Conjunctivitis; Staph-epidermidis; E. coli; black tea; vancomycin | ||
| References | ||
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