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Detection of some cytotoxin genes in local isolate of Oscillatoria (Cyanophyta) | ||
| Journal of University of Anbar for Pure Science | ||
| Volume 18, Issue 1, June 2024, Pages 26-31 PDF (888.38 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.37652/juaps.2023.143195.1129 | ||
| Authors | ||
| safa ghsaan Hameed; Harith kamil Buniya* | ||
| Department of Biology, College of Education for pure sciences, University Of Anbar, Ramadi,Iraq | ||
| Abstract | ||
| The cyanobacteria, included Ocsillatoria can produce different types of toxin called cyanotoxins. which is a wide range of structurally diverse and biologically active in freshwater, marine and terrestrial ecosystems. This study aimed to detect the some cyanotoxin genes in local Oscillatoria species to know their able to produce toxins. The current study included the isolation and purification of seven local freshwater isolates of the genus Oscillatoria from Ramadi city, Anbar Governorate, western Iraq. that were detected microscopically, these species are: O. acuta , O. princeps Vaucher ,O. annae , O. margaritifera ,O. proteus Skuja, O. sp. ,O. sancta. the unialgal then axenic culture prepared using BG11 medium to get algal biomass. After the DNA extraction, by specific primers for some cyanotoxin genes with conventional PCR, was used to detect a group of selected genes, namely mcy. E, ana.X, Ana.C, Ana. D and HEP. The results showed the presence of these genes in local isolates, Which indicates the ability of the studied isolates to produce these types of toxins. The presented results indicate the potential threat to many organisms in the ecosystem, including animals and humans, as the results showed the presence of hepatoxin genes in five types of studied samples, and the presence of neurotoxins in four types of studied samples. | ||
| Keywords | ||
| Cyanobacteria,,; ,،Oscillatoria,,; ,،cyanotoxins,,; ,،harmful algal bloom | ||
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