H. F. Al-Fahdawi, A., A. T. Al-Fahdawi, W. (2024). EFFECT OF GLYCINE BETAINE ON SOME BIOCHEMICAL TRAITS OF MUNG BEAN UNDER WATER STRESS CONDITIONS. , 22(1), 572-583. doi: 10.32649/ajas.2024.183757
A. H. F. Al-Fahdawi; W. A. T. Al-Fahdawi. "EFFECT OF GLYCINE BETAINE ON SOME BIOCHEMICAL TRAITS OF MUNG BEAN UNDER WATER STRESS CONDITIONS". , 22, 1, 2024, 572-583. doi: 10.32649/ajas.2024.183757
H. F. Al-Fahdawi, A., A. T. Al-Fahdawi, W. (2024). 'EFFECT OF GLYCINE BETAINE ON SOME BIOCHEMICAL TRAITS OF MUNG BEAN UNDER WATER STRESS CONDITIONS', , 22(1), pp. 572-583. doi: 10.32649/ajas.2024.183757
H. F. Al-Fahdawi, A., A. T. Al-Fahdawi, W. EFFECT OF GLYCINE BETAINE ON SOME BIOCHEMICAL TRAITS OF MUNG BEAN UNDER WATER STRESS CONDITIONS. , 2024; 22(1): 572-583. doi: 10.32649/ajas.2024.183757

EFFECT OF GLYCINE BETAINE ON SOME BIOCHEMICAL TRAITS OF MUNG BEAN UNDER WATER STRESS CONDITIONS

Anbar Journal of Agricultural Sciences
Volume 22, Issue 1, June 2024, Pages 572-583    PDF (555.51 K)
Document Type: Research Paper
DOI: 10.32649/ajas.2024.183757
Authors
A. H. F. Al-Fahdawi; W. A. T. Al-Fahdawi
Department of field crops, College of Agriculture, University of Anbar
Abstract
A field experiment was carried out during the spring season of 2022 at Research Station No.2, College of Agriculture, University of Anbar. The experiment followed a randomized complete block design (RCBD) in a split-plot arrangement with three replications to study the effect of four concentrations of Glycine Betaine acid (0, 50, 100, 150 mg L-1), denoted as GB2, GB3, and GB4 respectively, on some biochemical traits of mung bean under water stress conditions. The water stress levels were S1 (irrigated with 100% of available water, serving as the control treatment), and S2, S3, and S4, representing depletion rates of 25%, 50%, and 75% of available water, respectively. The results showed that the GB4 concentration was superior in total chlorophyll content in leaves (1.677 mg g-1), relative water content (55.7%), proline content (124.74 µg g-1), catalase enzyme activity (22.84 units ml-1), and peroxidase enzyme activity (28.42 units ml-1). The water stress treatment S1 excelled in giving the highest values for total chlorophyll content in leaves (1.842 mg g-1), relative water content (60.9%), proline content (144.38 µg g-1), catalase enzyme activity (29.69 units ml-1), and peroxidase enzyme activity (34.07 units ml-1). The interaction between water stress level S1 and GB4 concentration resulted in the highest rates for total chlorophyll content in leaves (1.930 mg g-1), relative water content (67.2%), proline content (186.30 µg g-1), catalase enzyme activity (35.13 units ml-1), and peroxidase enzyme activity (35.19 units ml-1).
Keywords
Glycine betaine; Water stress; Mung bean; CAT; POD; Proline
References
  1. Aebi, H. (1984). Catalase in vitro. In Methods in enzymology, 105: 121-126. https://doi.org/10.1016/S0076-6879(84)05016-3.
  2. Abdulilah, H.A.Q., Ali Abed Al-Asafi, E., and Hameed, A.T. (2019). Role of rhizobia (Rhizobium meliloti ) of alfalfa in the bioremediation. of contaminated soil with hydrocarbons.Plant Archives, 19: 146-152
  3. Adegoye, G. A., Olorunwa, O. J., Alsajri, F. A., Walne, C. H., Wijewandana, C., Kethireddy, S. R., ... and Reddy, K. R. (2023). Waterlogging effects on soybean physiology and hyperspectral reflectance during the reproductive stage. Agriculture, 13(4): 844.
  4. AL-Fahdawi, A. I. H. (2004). Effect of spraying with potassium and phosphate fertilization on some growth characteristics, yield and quality traits of several genotypes mung bean. Vigna radiata L. Master thesis faculty of Agriculture. Anbar University.
  5. Allahmoradi, P., Ghobadi, M., Taherabadi, S., and Taherabadi, S. (2011). Physiological aspects of mungbean (Vigna radiata L.) in response to drought stress. In International conference on food engineering and biotechnology, 9: pp. 272-275.
  6. Al-Sulaiman, A. M. M. (2014). The effect of Glycine Betain on Grain yield and its component and their heritability in its component and their heritability in bread wheat (Triticum aestivum L.). College of Basic Education Research Journal, 13(1): 895-906.
  7. Ashraf, M., and Foolad, M. R. (2007). Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and experimental botany, 59(2): 206-216. https://doi.org/10.1016/j.envexpbot.2005.12.006.
  8. Awad, H. O. (2009). Inheriting and breeding crops for stress (drought – high temperature – environmental pollution) part one. Egyption Library for printing, pubishing and Distribution, Alexandria, Arab Republic of Egypt.
  9. Bates, L. S., Waldren, R. P. A., and Teare, I. D. (1973). Rapid determination of free proline for water-stress studies. Plant and soil, 39: 205-207. https://doi.org/10.1007/BF00018060.
  10. Chen, T. H., and Murata, N. (2002). Enhancement of tolerance of abiotic stress by metabolic engineering of betaines and other compatible solutes. Current opinion in plant biology, 5(3): 250-257. https://doi.org/10.1016/S1369-5266(02)00255-8.
  11. Craig, S. A. (2004). Betaine in human nutrition. The American journal of clinical nutrition, 80(3): 539-549. https://doi.org/10.1093/ajcn/80.3.539.
  12. Goodwin, T. W. (1976). Chemistry and Biochemistry of Plant Pigment. 2nd Academic. Press. London, New York. San Francisco: 373.
  13. Hajim, A. Y. (2000). Water harvesting and supplemental irrigation. Iraqi Agriculture Journal. The third issue, pp.40-49. Ministry of Agriculture, Baghdad, Iraq.
  14. Jalil, P. A. (2023). First Mitochondrial Genome Report of Trichoferus Fissitarsis Sama, Fallahzadeh and Rapuzzi 2005 (Coleoptera: Cerambycidae) With Morphological Implications. Anbar Journal of Agricultural Sciences, 21(2): 354-365. doi: 10.32649/ajas.2023.142348.1067
  15. Lalinia, A. A., Hoseini, N. M., Galostian, M., Bahabadi, S. E., and Khameneh, M. M. (2012). Echophysiological impact of water stress on growth and development of mungbean. International Journal of Agronomy and Plant Production, 3(12): 599-607.
  16. Lichtenthaler, H. K. (1987). Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. In Methods in enzymology, 148: 350-382. https://doi.org/10.1016/0076-6879(87)48036-1.
  17. Ludlow, M. M., Santamaria, J. M., and Fukai, S. (1990). Contribution of osmotic adjustment to grain yield in Sorghum bicolor (L.) Moench under water-limited conditions. II. Water stress after anthesis. Australian Journal of Agricultural Research, 41(1): 67-78. https://doi.org/10.1071/AR9900067.
  18. Mackinney, G. (1941). Absorption of light by chlorophyll solutions. Journal of biological chemistry, 140(2): 315-322. https://doi.org/10.1016/S0021-9258(18)51320-X.
  19. Mafakheri, A., Siosemardeh, A. F., Bahramnejad, B., Struik, P. C., and Sohrabi, Y. (2010). Effect of drought stress on yield, proline and chlorophyll contents in three chickpea cultivars. Australian journal of crop science, 4(8): 580-585.
  20. Müftügil, N. (1985). The peroxidase enzyme activity of some vegetables and its resistance to heat. Journal of the Science of Food and Agriculture, 36(9): 877-880. https://doi.org/10.1002/jsfa.2740360918.
  21. Pitotti, A., Elizalde, B. E., and Anese, M. (1994). Effect of caramelization and Maillard reaction products on peroxidase activity. Journal of Food Biochemistry, 18(6): 445-457. https://doi.org/10.1111/j.1745-4514.1994.tb00515.x.
  22. Turner, N. C. (1981). Techniques and experimental approaches for the measurement of plant water status. Plant and soil, 58: 339-366. https://doi.org/10.1007/BF02180062.
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