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Anti-cancer activity of Carnosic acid against cervical carcinoma cell line through Catalase activity and TGF-β expression | ||
| The Medical Journal of Basrah University | ||
| Article 12, Volume 42, Issue 1, June 2024, Pages 91-99 PDF (567.61 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.33762/mjbu.2024.146927.1196 | ||
| Authors | ||
| Yousif Badr Makki1; Amir Norouzy2; Nazanin Jabbari* 3 | ||
| 1Department of Biology, Faculty of Convergent Sciences and Technologies, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 2Bioprocess Engineering Department, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran | ||
| 3Science And Research Branch, Daneshgah Blvd, Simon Bolivar Blvd, Tehran, Iran | ||
| Abstract | ||
| Background: Nowadays, the use of herbal medicines is very substantial in the treatment of cancer. One of the most common cancers among women is Cervical cancer. Due to the side effects of some chemical drugs, the use of medicinal plants with minimum side effects and interference has been considered. Rosemary (R. Official L.), a member of the Lamiaceae family, is known for its antioxidant properties. One of the main compounds for antioxidant activity is Carnosic acid (CA), a polyphenol with antitumor biological activities. Method: This study investigated the effect of Carnosic acid at different concentrations (2.5, 5, 10, 15, 20, 25, and 50) mg/ml on a Cervical cancer cell line (Hela). Cytotoxicity and growth inhibitory power (IC50) were calculated using the MTT test, Prism software. The real-time PCR method investigated TGF-β1 gene expression on Hela cancer cell lines and cells treated with Carnosic acid. To assess and quantify oxidative stress and apoptotic behavior, we utilized a plate reader and the appropriate kit to measure catalase activity (CAT) level by detecting 570 nm light absorption. Results: Cytotoxicity assay (MTT) showed the antitumor activity of the group treated with CA compared to the control group (Hela cells without treatment). Gene expression analysis based on RT-PCR showed downregulation of the TGF-β gene and catalase activity reduction in cells treated with Carnosic acid compared to untreated cells. Conclusion: Carnosic acid has been introduced as a promising formula for treating cervical cancer. | ||
| Keywords | ||
| Keywords: Carnosic acid; Catalase; Cervical Cancer; HeLa Cell; Oxidative Stress; TGF-β | ||
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