Molecular Prevalence and Genotyping of Toxoplasma gondii from Pet Cat Using PCR-RFLP

Ahmed, Falah H; Jori, Muna; Al-Azizz, Suzan Abduljabbar

doi:10.23975/bjvr.2024.147020.1061

	Molecular Prevalence and Genotyping of Toxoplasma gondii from Pet Cat Using PCR-RFLP
Basrah Journal of Veterinary Research
Volume 23, Issue 2, June 2024, Pages 45-53 PDF (416.03 K)
Document Type: Research Paper
DOI: 10.23975/bjvr.2024.147020.1061
Authors
Falah H Ahmed^* ¹; Muna Jori²; Suzan Abduljabbar Al-Azizz³
¹Department of microbiology and parasitology, college of veterinary medicine, university of Basrah, Basrah, Iraq
²department of Microbiology and Parasitology, college of Veterinary Medicine, university of basrah, Basrah, Iraq
³Department of Microbiology and Parasitology, college of veterinary medicine, university of basrah, Basrah , Iraq
Abstract
Toxoplasma gondii is the most dangerous parasite found in humans and cats. The strong relationship between cats and their owners compelled us to explore the incidence of these parasites in companion cats and genotyping of T.gondii strain of the infected pets. 54 blood samples and 54 fecal samples collected from pets in order to ascertain the prevalence of T. gondii. The GRA6 gene was targeted in the feces and blood of examined pets and the genotypes of T. gondii were determined using the GRA6 gene loci by PCR-RFLP assay. The GRA6 gene amplification showed that 6% of pets were infected with T.gondii. PCR-RFLP assay revealed the predominance of genotype II at a rate 67% and type III at a rate 33% while type I not detected in infected pets. The incidence of toxoplasmosis in domestic felines can cause contamination of environment through excreting of oocyst in the feces which may lead to infection to cat owners specially who taking care of feeding and cleaning of pets.
Keywords
Toxoplasmosis; PCR; GRA; pet cat; genotyping

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