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Screening and Molecular Dynamic Simulations of Colicin Produced by Commensals Escherichia coli Isolated from Animals | ||
| Basrah Journal of Veterinary Research | ||
| Volume 23, Issue 2, June 2024, Pages 65-72 PDF (671.19 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.23975/bjvr.2024.150613.1098 | ||
| Authors | ||
| Amal F. Ghanim1; Basil Abbas* 1; Ali B. Al-Deewan1; Hanan Hasan Ali2 | ||
| 1Department of Microbiology, College of Veterinary Medicine, University of Basrah, Iraq | ||
| 2College of Health and Sciences, North Carolina Central University, Durham, United States of America | ||
| Abstract | ||
| E. coli strains isolated from animal feces were subjected to detection of colicin production. The produced colicin was prepared as a disc to serve as an inhibitor for other types of bacteria on Mueller-Hinton agar. The result indicates that the produced colicin has an inhibition effect against Staphylococcus sp. with inhibition zone vary from 1.3 to 2.5 cm. Moreover, the result of electrophoresis of total crude protein after cell lysis with sonication verify the presence of 130kD bands in the supernatant. The results of docking were analyzed and compared to predict the potential binding affinities of the colicin types against the FH VH at lipid П protein. | ||
| Keywords | ||
| Colicin; Escherichia coli; docking | ||
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