Ayob, S., Saleh, O., Alhussary, B., Taqa, G. (2024). The Antioxidative Role of Moringa Oil extract in Modulating Histological and Biochemical Changes in the Salivary Glands of Rats Under Oxidative Stress Induction. , 21(4), 136-145. doi: 10.33899/iraqij.p.2024.150109.1101
Shemaa A. Ayob; Omer W. Saleh; Banan N. Alhussary; Ghada A. Taqa. "The Antioxidative Role of Moringa Oil extract in Modulating Histological and Biochemical Changes in the Salivary Glands of Rats Under Oxidative Stress Induction". , 21, 4, 2024, 136-145. doi: 10.33899/iraqij.p.2024.150109.1101
Ayob, S., Saleh, O., Alhussary, B., Taqa, G. (2024). 'The Antioxidative Role of Moringa Oil extract in Modulating Histological and Biochemical Changes in the Salivary Glands of Rats Under Oxidative Stress Induction', , 21(4), pp. 136-145. doi: 10.33899/iraqij.p.2024.150109.1101
Ayob, S., Saleh, O., Alhussary, B., Taqa, G. The Antioxidative Role of Moringa Oil extract in Modulating Histological and Biochemical Changes in the Salivary Glands of Rats Under Oxidative Stress Induction. , 2024; 21(4): 136-145. doi: 10.33899/iraqij.p.2024.150109.1101

The Antioxidative Role of Moringa Oil extract in Modulating Histological and Biochemical Changes in the Salivary Glands of Rats Under Oxidative Stress Induction

Iraqi Journal of Pharmacy
Article 1, Volume 21, Issue 4, December 2024, Pages 136-145    PDF (2.11 M)
Document Type: Research Paper
DOI: 10.33899/iraqij.p.2024.150109.1101
Authors
Shemaa A. Ayob; Omer W. Saleh; Banan N. Alhussary; Ghada A. Taqa*
Department of Basic Sciences, College of Dentistry, University of Mosul, Mosul, Iraq
Abstract
Background: Medicinal plants are a rich source of antioxidants, and attention has been drawn to them in subsequent years, as the Moringa plant is one of these sources. The aim of this work is to identify the protective role of Moringa oil versus the oxidative stress induced by H2O2 in the salivary glands of rats. Materials methods: 27 rats  were divided  into 3 groups. A daily dose of 1000 mg/kg of moringa oil was used for the first group. The second group received a daily dosage of 2 ml/rat of 0.5% H2O2. For 21 days, the third group received a daily dosage of 1000 mg/kg of moringa oil and 2 ml/rat of 0.5% H2O2.  After 7, 14, and 21 days blood samples are drawn to measure TAC, GSH and MDA, and salivary gland samples are taken for histological examination. Results:  There are significant differences in TAC, GSH and MDA levels between the treatment groups within each period and significant variations across different periods within the same treatment group. The histological examination suggests that the Moringa oil group generally maintained normal glandular architecture, while the H₂O₂ group exhibited signs of degeneration and necrosis. The Moringa + H2O2 group showed a protective effect in some instances, preserving tissue structure. Conclusion: the findings imply that Moringa oil could offer protection against oxidative stress by influencing TAC levels and reducing MDA levels. The combination of H2O2 and Moringa oil seems to counterbalance the impact of hydrogen peroxide. Moreover the group using Moringa oil generally maintained a structure while the H2O2 group showed signs of deterioration and cell death. The group using both Moringa oil and H2O2 exhibited some effects at times preserving the tissue structure of the gland.
Keywords
Antioxidant; Moringa; H₂O₂; Histopathology; Salivary gland
References
  1. Engwa GA, Nweke FN, Nkeh-Chungag BN. Free radicals,oxidative stress-related diseases and antioxidant supplementation. Alternative Therapies in Health & Medicine 2022;28(1).
  2. Mitra AK. Antioxidants: a masterpiece of mother nature to prevent illness. Journal of Chemical Reviews. 2020;2(4):243-56.
  3. Sutkowy P, Woźniak A, Mila-Kierzenkowska C, Szewczyk-Golec K, Wesołowski R, Pawłowska M, Nuszkiewicz J. Physical activity vs. redox balance in the brain: brain health, aging and diseases. Antioxidants 2021;11(1):95
  4. Alhalawachee Taqa, GA. Sonographic Assessment of Submandibular Salivary Glands in Hypothyroid Rat’s Pups Before and After Ashwagandha Root Extract Treatment. Iraqi Journal of Pharmacy 2023; 20(1): 1-6.‏
  5. Jacques AS, Arnaud SS, Jacques DT. Review on biological and immunomodulatory properties of Moringa oleifera in animal and human nutrition. Journal of Pharmacognosy and Phytotherapy 2020;12(1):1-9.
  6. Ercan K, Gecesefa OF, Taysi ME, Ali Ali OA, Taysi S. Moringa oleifera: a review of its occurrence, pharmacological importance and oxidative stress. Mini Reviews in Medicinal Chemistry 2021;21(3):380-96.
  7. Islam MA, Haque MA, Rahman MA, Hossen F, Reza M, Barua A, Marzan AA, Das T, Kumar Baral S, He C, Ahmed F. A review on measures to rejuvenate immune system: Natural mode of protection against coronavirus infection. Frontiers in Immunology 2022;13:837290.
  8. Athikomkulchai S, Tunit P, Tadtong S, Jantrawut P, Sommano SR, Chittasupho C. Moringa oleifera seed oil formulation physical stability and chemical constituents for enhancing skin hydration and antioxidant activity. Cosmetics 2020;8(1):2.
  9. Mustafa YF, Waheed SA, Jasim SF, Jebir RM, Ismael RN, Qutachi O. A Narrative Review of Benzo-Fused Coumarins, Shedding Light on Their Medicinal Activities. Iraqi Journal of Pharmacy2023;20(1):7–16.
  10. Urakov AL, Urakova N, Fisher EL, Yagudin I, Darya S, Svetova M, Shubina Z, Muhutdinov N. Inhalation of an aerosol solution of hydrogen peroxide and sodium bicarbonate for the urgent recanalization of the respiratory tract after blockage by mucus and pus. Journal of Modern Biology and Drug Discovery 2022;1(2):1-4.
  11. Spolverato YC, Zuin M, Valmasoni M, Fiscon V, Portale G. Hydrogen Peroxide H₂O₂ Poisoning: Air in the Liver. Indian Journal of Surgery 2022;84(3):565-7.
  12. Nilsson R, Liu NA. Nuclear DNA damages generated by reactive oxygen molecules (ROS) under oxidative stress and their relevance to human cancers, including ionizing radiation-induced neoplasia part I: physical, chemical and molecular biology aspects. Radiation Medicine and Protection 2020;1(3):140-52.
  13. Mustafa YF, Abdulaziza NT, Jasima MH. 4-Methylumbelliferone and its derived compounds: A brief review of their cytotoxicity. Egyptian Journal of Chemistry2021;64(4):1807–16.
  14. Pravda J. Hydrogen peroxide and disease: towards a unified system of pathogenesis and therapeutics. Molecular Medicine 2020;26(1):1-0.
  15. Díaz Y, Tabío D, Rondón M, Piloto-Rodríguez R, Fernández E. Phenomenological model for the prediction of Moringa oleifera extracted oil using a laboratory Soxhlet apparatus. Grasas y Aceites 2021;72(3):e422.
  16. Mihailidis TH. The use of hydrogen peroxide in the treatment of burn wound infection: a systematic review, and survey of current clinical practice in the United Kingdom. International Journal of Burns and Trauma 2020;10(2):38.
  17. Senthilkumar A, Thangamani A, Karthishwaran K, Cheruth AJ. Essential oil from the seeds of Moringa peregrina: Chemical composition and antioxidant potential. South African Journal of Botany 2020;129:100-5.
  18. Shoaib M, Kim N, Choudhary RC, Yin T, Shinozaki K, Becker LB, Kim J. Increased plasma disequilibrium between pro-and anti-oxidants during the early phase resuscitation after cardiac arrest is associated with increased levels of oxidative stress end-products. Molecular Medicine 2021;27(1):1-3.
  19. Sreelatha, S.; Padma, P.R. Antioxidant Activity and Total Phenolic Content of Moringa oleiferaLeaves in Two Stages of Maturity. Plant Foods for Human Nutrition 2009; 64: 303–311.
  20. Alvariño, R.; Alonso, E.; Alfonso, A.; Botana, L.M. Neuroprotective Effects of Apple-Derived Drinks in a Mice Model of Inflammation. Molecular Nutrition & Food Research 2019, 64: 1901017.
  21. Nair, D.A.; Joseph, J.; Sreelatha, S.; Kariyil, J.; Nair, S. Moringa oleifera (Lam.): A natural remedy for ageing? Natural Product Research 2020; 23: 1–7.
  22. Chandrasekhar, Y.; Kumar, G.P.; Ramya, E.M.; Anilakumar, K.R. Gallic Acid Protects 6-OHDA Induced Neurotoxicity by Attenuating Oxidative Stress in Human Dopaminergic Cell Line. Neurochemical Research 2018; 43: 1150–1160.
  23. González-Sarrías, A.; Núñez-Sánchez, M.Á.; Tomás-Barberán, F.A.; Espín, J.C. Neuroprotective Effects of Bioavailable Polyphenol-Derived Metabolites against Oxidative Stress-Induced Cytotoxicity in Human Neuroblastoma SH-SY5Y Cells. Journal of Agricultural and Food Chemistry2017; 65: 752–758.
  24. Yu, M.; Chen, X.; Liu, J.; Ma, Q.; Zhuo, Z.; Chen, H.; Zhou, L.; Yang, S.; Zheng, L.; Ning, C.; et al. Gallic acid disruption of Aβ1–42 aggregation rescues cognitive decline of APP/PS1 double transgenic mouse. Neurobiology of Disease 2019; 124: 67–80. 
  25. Mohammed ET, Mustafa YF. Coumarins from Red Delicious apple seeds: Extraction, phytochemical analysis, and evaluation as antimicrobial agents. Systematic Reviews in Pharmacy2020;11(2):64–70.
  26. Liang LL, Cai SY, Gao M, Chu XM, Pan XY, Gong KK, Xiao CW, Chen Y, Zhao YQ, Wang B, Sun KL. Purification of antioxidant peptides of Moringa oleifera seeds and their protective effects on H₂O₂ oxidative damaged Chang liver cells. Journal of Functional Foods 2020;64:103698.
  27. Hussein HM, Taqa, GA. Protective Effect of Moringa on Salivary Glands of Rats Exposed to Electromagnetic Radiation of Mobile Phone: A Histological Study. Journal of Applied Veterinary Sciences 2023; 8(3):30-35.‏
  28. Alnuaimi SI, Alabdaly YZ. Neurobehavioral toxicity of copper sulfate accompanied by oxidative stress and histopathological alterations in chicks' brain. Iraqi Journal of Veterinary Sciences 2023;37(1):53-60.
  29. Akbari B, Baghaei-Yazdi N, Bahmaie M, Mahdavi Abhari F. The role of plant-derived natural antioxidants in reduction of oxidative stress. BioFactors 2022;48(3):611-33.
  30. Akbari B, Baghaei‐Yazdi N, Bahmaie M, Mahdavi Abhari F. The role of plant-derived natural antioxidants in reduction of oxidative stress. BioFactors 2022;48(3):611-33.
  31. Ercan K, Gecesefa OF, Taysi ME, Ali Ali OA, Taysi S. Moringa oleifera: a review of its occurrence, pharmacological importance and oxidative stress. Mini Reviews in Medicinal Chemistry 2021;21(3):380-96.
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