MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF POSTHARVEST FUNGAL PATHOGEN (<i>Penicillium chrysogenum</i>) IN POMEGRANATE FRUITS (<i>Punica granatum</i> L.) IN DUHOK PROVENCE- IRAQ

Sinjari, Dalal Yousif; Abdullah, Shahad Najemadeen; Jubrael, Jaladet Mohammed; Musa, Vian Majeed

doi:10.33899/mja.2024.148152.1400

	MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF POSTHARVEST FUNGAL PATHOGEN (Penicillium chrysogenum) IN POMEGRANATE FRUITS (Punica granatum L.) IN DUHOK PROVENCE- IRAQ
Mesopotamia Journal of Agriculture
Article 7, Volume 52, Issue 3, September 2024, Pages 106-117 PDF (429.92 K)
Document Type: Research
DOI: 10.33899/mja.2024.148152.1400
Authors
Dalal Yousif Sinjari¹; Shahad Najemadeen Abdullah^* ²; Jaladet Mohammed Jubrael¹; Vian Majeed Musa¹
¹Scientific Research Center, College of Science, University of Duhok, Duhok, Iraq
²Plant Protection, College of Agriculture Engineering Sciences, University of Duhok, Duhok, Iraq
Abstract
The research aimed to identify and characterize six samples of P. chrysogenum isolated from pomegranate fruits in Duhok City – Iraq. The study used morphological, internal transcribed spacer (ITS) region anitsd PCR-RFLP markers. The morphological examination revealed consistent characteristics across all six samples, indicating their classification as P. chrysogenum. The macroscopic features showed dense, blue-green colonies with a velvety texture, revealing brush-like conidiophores and distinctive conidia structures. Molecular analysis of the ITS region showed an approximately 600bp band that confirmed the morphological identification, providing additional support for the presence of P. chrysogenum in the pomegranate samples. The obtained sequences using 28S rRNA partial gene of 1200bp showed a high degree of similarity with known P. chrysogenum reference sequences, further validating the identification. Furthermore, the enzymes Rsa I, Hae III, and Alu I were chosen to study the restriction site amplification of the affected ITS region of all examined P. chrysogenum. The results showed significant identical amplified bands among the studied isolates. This study may pave the way for the distribution of P. chrysogenum pathogens in Duhok City and highlights the importance of integrating molecular and morphological techniques for accurate identification.
Keywords
PCR- RFLP; penicillium chrysogenum; pomegranate; sequence (punica granatum L.) OR226321

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MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF POSTHARVEST FUNGAL PATHOGEN (Penicillium chrysogenum) IN POMEGRANATE FRUITS (Punica granatum L.) IN DUHOK PROVENCE- IRAQ