Formation of Genetically Transformed Hair Roots of Chamomile (Matricaria chamomilla L.,) Plants By Ri-Plasmid Vector of Agrobacterium rhizogenes ATCC 15834 Molecular

Ibraheim, Aala Moath; Mohammed, Amjad Abdul-Hadi

doi:10.33899/edusj.2024.148175.1

	Formation of Genetically Transformed Hair Roots of Chamomile (Matricaria chamomilla L.,) Plants By Ri-Plasmid Vector of Agrobacterium rhizogenes ATCC 15834 Molecular
Journal of Education and Science
Volume 33, Issue 3, September 2024, Pages 78-84 PDF (749 K)
Document Type: Research Paper
DOI: 10.33899/edusj.2024.148175.1
Authors
Aala Moath Ibraheim^* ; Amjad Abdul-Hadi Mohammed
Department of Biology/ College of Science/ University of Mosul/ Mosul/ Iraq.
Abstract
This study succeeded in developing hairy roots from the hypocotyledonous stems of chamomile Matricaria chamomilla L. plant seedlings by Ri plasmid isolated from Agrobacterium rhizogenes ATCC 15834 using direct injection technology, and exceeding the concentration 1214.32 In encouraging the development of hair roots by 80% after7 day. After isolating the hair roots from their places of formation and placing them individually or as tufts on solid Murashige and Skoog (MS) medium, typical cultures were produced and required subcultured every 25 One day, the hair roots were characterized by being white, branched, and growing negative geotrpism. The results of electrophoresis on an agarose gel of the isolated polymerase chain reaction (PCR) product, which included the amplification of deoxyribonucleic acid (DNA), showed the appearance of a single band with a molecular size of 248 bp, which is similar to the molecular size of the specific primer of the rol A gene, and this is evidence of the success of the transformation. Genetic analysis by transferring T-DNA genes from plasmids and inserting them into the cell genome of seedlings of the chamomile plant.
Keywords
Chamomile plant; Genetic transformation; Plasmid vector Ri; PCR

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