Polymerase Chain Reaction for the Detection of Histamine-Producing Histophilus somni Isolated from Basra Cattle

Jebur, Raghad M.; Abdullah, Fawzia A.; Mustafa, Jalal Y.

doi:10.23975/bjvr.2024.149689.1086

	Polymerase Chain Reaction for the Detection of Histamine-Producing Histophilus somni Isolated from Basra Cattle
Basrah Journal of Veterinary Research
Volume 23, Issue 2, June 2024, Pages 149-161 PDF (492.72 K)
Document Type: Research Paper
DOI: 10.23975/bjvr.2024.149689.1086
Authors
Raghad M. Jebur; Fawzia A. Abdullah; Jalal Y. Mustafa
College of Veterinary Medicine, University of Basrah
Abstract
This study sought to examine the significance of Histophilus somni as a producer of histamine and a respiratory tract pathogen. Conventional bacteriological and molecular techniques were used to examine 200 nasal swab samples for the presence of Histophilus somni and its ability to produce histamine. Out of 200 cattle nasal swabs tested, 25% (50/200) tested positive for conventional biochemical properties and culture. Histophilus somni was found in 70% (35/50) of the isolates that tested positive for the PCR test after culturing. It was found that 20% (7/35) of the 35 H. somni positive PCR isolates produced positive products when the histidine decarboxylase gene was amplified. Cattle that were infected in the first age group (less than one year) had higher percentages of culture (72%; 36/50) and positive PCR results (80%; 28/35), based on their age. Males demonstrated higher culture (82%;41/50) and PCR (85%; 30/35) positivity percentages regarding the impact of sex. There was a statistically significant difference (P<0.05) between the genders and between the two age groups.
Keywords
PCR; Histophilus somni; Histamine

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