The effectiveness of fermented juices on weight gain and Microbial Balance in rats by Induced diarrhea with E.coli

Al-Hadidy, Yasmeen I.; Alnuame, Omer A.; Werdi, Chalang Madad

doi:10.58928/ku24.15303

	The effectiveness of fermented juices on weight gain and Microbial Balance in rats by Induced diarrhea with E.coli
Kirkuk University Journal for Agricultural Sciences (KUJAS)
Volume 15, Issue 3, September 2024, Pages 15-21 PDF (434 K)
Document Type: Research Paper
DOI: 10.58928/ku24.15303
Authors
Yasmeen I. Al-Hadidy¹; Omer A. Alnuame²; Chalang Madad Werdi^* ³
¹Department of Food Sciences/College of Agriculture, Tikrit University, Tikrit, Iraq.
²Department of Arabic Language, College of Basic Education, Al-Sharqat, Tikrit University, Tikrit, Iraq
³College of Veterinary Medicine / University of Kirkuk
Abstract
The current study aimed to evaluate the effectiveness of pomegranate and orange juices fortified with Probiotic Lb.plantarum, Lb. rhamnosus and Lb. acidophilus in weight gain and the balance of the natural intestinal flora in experimental animals by induced diarrhea infection with E. coli. 24 male rats (Albino Sprague – Dawley) were used and were divided into eight groups with three replicates, and the duration of the e experiment was 28 days. The results of the statistical analysis showed, with probability (P<0.05), that infection with diarrhea by E.coli caused a significant increase in the weights of the rats. The highest final weight gain in the infected male rats was (101.5) grams, and that treatment with oral administration of fermented juices led to a decrease in the weights animals: As for the treatment with fermented orange juice, the infected treatment and the treatment with orange juice fortified with Lb.plantarum outperformed the group of rats with diarrhea and the treatment with pomegranate juice. The amount of weight gain was 65 grams, and the initial and final weight were (173 and 238) grams, respectively. As for pomegranate juice, the group of rats with infection and treated with pomegranate juice outperformed. The fermented with the probiotic L .acidophilus, the amount of weight gain was 54 grams, and the initial and final weight were (184 and 238.5) grams, respectively. As for the balance of normal flora of the intestine, the results of the statistical analysis was showed, with (P<0.05), the effect of diarrhea caused with E.coli on the microbial balance of the intestines, as it caused a significant increase in the total count of coliform, Salmonella and Shigella, and a significant decrease in the total count of Lactic acid bacteria (LAB) compared to control groups and groups of infected and treated groups,
Keywords
Orange juice fortified with probiotics; pomegranate juice fortified with probiotics; E.coli infection; weight gain; the gut microbial balance

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