Influence of Auxins and different growths media strength on rooting of Petunia hybrida L. In vitro Propagation

Kamal, Taban Falah; Alsaad, Kefaia; Noori, Ali Mohammed

doi:10.58928/ku24.15315

	Influence of Auxins and different growths media strength on rooting of Petunia hybrida L. In vitro Propagation
Kirkuk University Journal for Agricultural Sciences (KUJAS)
Volume 15, Issue 3, September 2024, Pages 126-132 PDF (656.39 K)
Document Type: Research Paper
DOI: 10.58928/ku24.15315
Authors
Taban Falah Kamal^* ¹; Kefaia Alsaad²; Ali mohammed Noori³
¹Kirkuk university-college of agriculture-department of horticulutre
²Dept. of Horticulture and Landscaping Design\ College of Agriculture\ University of Kirkuk\Kirkuk\ IRAQ
³Dep. Horticulture, College of Agriculture, University of Kirkuk, Kirkuk, IRAQ
Abstract
This experiment was carried out in the Plant Cells and Tissue Culture Laboratory of Horticulture and Landscaping Design Department / College of Agriculture / University of Kirkuk - IRAQ, to study the effect of auxin’s in different concentrations and MS media (full and ½) salt strength on rooting response of Petunia hybrida. The regenerated shoots explants from the multiplication stage were transferred to the rooting stage cultured on MS media ( full and ½ ) salts strength supplemented with different concentrations of IBA at ( 0.0, 0.25, 0.50, 0.75, and 1.0 ) mg.L-1, and NAA at ( 0.0, 0.25, 0.50, 0.75, and 1.0 ) mg.L-1. After 6 weeks of transforming, data showed that, the response percentage was ( 100% ) for MS media ( full and ½ ) salt strength supplemented with different concentrations of IBA and NAA beside control treatment. The best number of roots ( 29.70 root.part-1 ) and the length of the longest root ( 7.44 cm ) were recorded by MS ½ strength supplemented with IBA (1.0 mg.L-1) compared with other concentrations, and it was the highest results compared with MS full strength supplemented with different concentration of IBA. Moreover, results obtained that MS ½ strength supplemented with NAA ( 1.0 mg.L-1 ) recorded the best number of roots ( 18.70 root.part-1 ) and the length of the longest root ( 2.97 cm ) compared with the other concentrations, while it was the best result compared with MS full strength supplemented with different concentrations of NAA.
Keywords
Auxin; Petunia hybrida; MS; In Vitro; IBA; NAA

References
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