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Selenium Enriched Yeast Modifies the Effects of Methandienone in Male Rabbits on the HPA Axis and Adrenal Gland Oxidative Stress | ||
| Al-Anbar Journal of Veterinary Sciences | ||
| Volume 13, Issue 2, March 2020, Pages 91-99 PDF (907.2 K) | ||
| Document Type: Research Paper | ||
| Authors | ||
| Sabea Khamees Abed* 1; Tahani S.S. Al-Azawi2 | ||
| 1Dept.of Physiology&Pharmacology, College of Veterinary Medicine, University of Fallujah, Iraq | ||
| 2College of Veterinary Medicine, University of Baghdad, Iraq | ||
| Abstract | ||
| The aim of this study was to investigate the changes caused by anabolic androgenic steroids (methandienone) in the hypothalamic adrenal axis (HPA) and oxidative status in adrenal glands and a protective role role by using organic selenium (high-selenium yeast) in adult male rabbits which treated with Methandienone in combination with selenium. A total of 20 intact male rabbits were divided into four equal groups (n = 5 for each group):a control group receiving distilled water, the AAS group (T1) receiving Methandienone (oral dose of 0.35 mg / kg.B.W.),the selenium group (T2) receiving high-selenium yeast (3 μg/ kg B.W orally)and the combination group (T3) getting both methandienone and selenium. The dosing every day for 60 days. . The hormones β-endorphin, ACTH and cortisone were measured in serum during two periods, 30 and 60 days of the experiment and the expression levels of Nuclear factor erythroid-2 and related factor-2 (Nrf2) and Translocater protein (TSPO) genes were measured in adrenal gland. a significant increase in beta-endorphin and ACTH and a decrease in cortisone were found in both 30 and 60-day periods in the AAS group (T1) compared with another group (control, T2 and T3). On the other hand, T3 group shows a decrease in serum β-Endorphin and ACTH and no significant changes in cortisone in both periods . These changes were compared with to AAS group. Moreover, there is a significant increase in revers transcription (mRNA) of Nrf2 factor and TSPO genes in AAS group(T1) as compared with other groups (T2 and T3). In addition, we observed a significant decrease in levels of Nrf2 and TSPO mRNA in the selenium-yeast group compared to the AAS group (T1). In conclusion, these results showed that organic selenium (high selenium yeast) has a positive effect (protective role) against oxidative damage to the adrenal gland caused by AAS with no effect on the adrenal steroidogenesis, which are affected by AAS. | ||
| Keywords | ||
| Anabolic androgenic steroids; Methandienone; Selenium; Rabbit | ||
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